1) DNA Direct PCR-sequencing method
DNA直接测序法
3) DNA direct sequencing
DNA直接测序
1.
Polymerase chain reaction and silver staining-single strand conformation polymorphism (PCR-SSCP) and DNA direct sequencing were used to detect the mutation of p53 gene in exons 5-8.
方法 应用聚合酶链反应 -单链构象多态性 (PCR- single strand conformation polymorphism,PCR- SSCP)银染技术结合 DNA直接测序 ,检测喉鳞癌新鲜或冰冻组织中 p5 3外显子 5～ 8的基因突变。
4) direct sequencing
直接测序法
1.
Method Select blood,heart,liver,spleen,lung,kidney,pancreas,brain,muscle,skin,rib,nail and hair of 18 individuals,screening the heteroplasmyic samples by DHPLC,and confirm by direct sequencing.
方法选取尸体18例,分别提取血、心、肝、脾、肺、肾、胰腺、脑、肌肉、皮肤、肋骨、指甲及毛发的mtDNA,用DHPLC筛选异质型样本,并用直接测序法进行验证。
2.
Samples were detected by direct sequencing and clone sequencing,and the results were compared.
目的:了解PCR产物直接测序法与克隆测序法在单核苷酸多态性(SNP)检测上的差别。
5) Direct PCR DNA sequence
PCR-DNA直接测序技术
6) direct sequencing
直接测序
1.
By means of PCR and direct sequencing,all 51 exons and their neighbouring intronic sequences of the COL4A5 gene were analyzed to detect mutations in 17 members from a Chinese family with X-linked Alport syndrome(XLAS).
通过PCR和直接测序的方法 ,对一性连锁Alport综合征家系 17个受检个体的COL4A5基因所有 5 1个外显子及其相邻内含子的DNA序列进行检测。
2.
The amplified specific sequence of the bovine mtDNA from meat and feed samples is demonstrated by both direct sequencing and restriction endonuclease digestion yield analysis.
样品的PCR产物经直接测序及限制性酶切片段分析 ,以进一步鉴定扩增结果。
3.
Methods By using immunohistochemical fechnique, MDT-PCR-SSCP and direct sequencing the mP53 expression and point muta- tion in exon 6 of p53 gene in both Solt-Farber model and DEN-induced liver cancer model were detected.
方法 通过ＤＥＮ诱发的启动模型和肝癌模型，应用免疫组化、ＭＤＴ－ＰＣＲ－ＳＳＣＰ和直接测序技术分别研究ｍＰ５３表达和 ｐ５３基因第６外显子点突变。
补充资料：直接测热法
直接测热法（direct calorimetry）：
将被测者置于一特殊的检测环境中，收集被测者在一定时间内（通过辐射、传导、对流及蒸发4个方面）发散的总热量，然后换算成单位时间的代谢量，即能量代谢率。直接测热的装置较为复杂，主要用于研究肥胖和内分泌系统障碍等。研究能量代谢一般采用间接测热法。
是能量需要量的测定方法中的一种。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条