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1)  Graded Direct Method
循序直接法
2)  direct sequencing
直接测序法
1.
Method Select blood,heart,liver,spleen,lung,kidney,pancreas,brain,muscle,skin,rib,nail and hair of 18 individuals,screening the heteroplasmyic samples by DHPLC,and confirm by direct sequencing.
方法选取尸体18例,分别提取血、心、肝、脾、肺、肾、胰腺、脑、肌肉、皮肤、肋骨、指甲及毛发的mtDNA,用DHPLC筛选异质型样本,并用直接测序法进行验证。
2.
Samples were detected by direct sequencing and clone sequencing,and the results were compared.
目的:了解PCR产物直接测序法与克隆测序法在单核苷酸多态性(SNP)检测上的差别。
3)  DNA Direct PCR-sequencing method
DNA直接测序法
4)  Direct cycle
直接循环
5)  progressive multiplication
循序乘法
6)  direct sequencing
直接测序
1.
By means of PCR and direct sequencing,all 51 exons and their neighbouring intronic sequences of the COL4A5 gene were analyzed to detect mutations in 17 members from a Chinese family with X-linked Alport syndrome(XLAS).
通过PCR和直接测序的方法 ,对一性连锁Alport综合征家系 17个受检个体的COL4A5基因所有 5 1个外显子及其相邻内含子的DNA序列进行检测。
2.
The amplified specific sequence of the bovine mtDNA from meat and feed samples is demonstrated by both direct sequencing and restriction endonuclease digestion yield analysis.
样品的PCR产物经直接测序及限制性酶切片段分析 ,以进一步鉴定扩增结果。
3.
Methods By using immunohistochemical fechnique, MDT-PCR-SSCP and direct sequencing the mP53 expression and point muta- tion in exon 6 of p53 gene in both Solt-Farber model and DEN-induced liver cancer model were detected.
方法 通过DEN诱发的启动模型和肝癌模型,应用免疫组化、MDT-PCR-SSCP和直接测序技术分别研究mP53表达和 p53基因第6外显子点突变。
补充资料:对比剂的直接引入法


对比剂的直接引入法


X线检查方法之一。将对比剂直接引入欲观察的器官或结构的造影检查方法。对比剂可通过人体自然腔道开口引入,或通过病理性腔道引入,还可采用穿刺的方法引入。通常在同一器官可以选择不同的直接引入法,如口服法、灌注法、顺行性或逆行性灌注法等。
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