2) Gel electrophoresis
凝胶电泳
1.
Single cell gel electrophoresis is applied for genetic toxicology,DNA damage and detection,environmental pollution detection,apoptosis mechanism research et al.
单细胞凝胶电泳被广泛应用于遗传毒理学、DNA损伤与修复的检测、环境污染检测、细胞凋亡机制的研究等诸多领域。
2.
In general, those problems such as the dim and bending band, strong fluorescence background, bad repetition often occured during DNA agarose gel electrophoresis.
针对琼脂糖凝胶电泳中经常出现的电泳图谱条带模糊、弯曲 ,图谱背景荧光较强 ,凝胶胶面不平以及重复性差等问题 ,提出了相应的简便可行的解决方
3.
The application of molecular methods such as hybridiaztion of nucleic acid probes, amplification of polymerase chain reaction (PCR), latest gel electrophoresis, quinone profiles and analyses of ribosomal RNA will extend the research field of microbial ecology, and make it possible to study the mechanism of ecology problem at molecular level.
本文介绍了在环境微生物生态研究中的分子生物学方法如分子杂交 ,聚合链式扩增技术PCR ,rRNA基因同源分析法 ,新型凝胶电泳技术 ,生物醌谱图法等和应用。
3) SDS-PAGE
凝胶电泳
1.
Selenoproteins in soybean from Ensh area where selenium in soil was at a higher level were seperated by using sodium dodecyl sulfste-polyacrylamide gel electrophoresis (SDS-PAGE) and then identified by using high Performance liquid chromatography with a fluorescent indication detector (HPLC-FID).
采用凝胶电泳技术分离恩施高硒地区大豆中的含硒蛋白组分，分辨出２７条蛋白条带。
2.
SDS-PAGE results showed there were 4 types of protein zones.
SDS凝胶电泳结果表明牡蛎含有4种主要蛋白区带,平均相对分子质量分别为16172,17633,43227,57909。
3.
UV and SDS-PAGE analysis suggest that the extraction product is typeⅠcollagen,and its molecule mass is about 320 kDa.
采用较简便的酶法从猪皮中提取胶原蛋白,通过SDS-PAGE凝胶电泳、紫外光谱定性分析,证明所提取胶原蛋白为Ⅰ型胶原蛋白,计算得分子量约为320 kD。
4) electrophoresis
[英][i,lektrəufə'ri:sis] [美][ɪ,lɛktrofə'risɪs]
凝胶电泳
1.
SDS electrophoresis confirmed the antigen protein was effectively degraded.
通过十二烷基磺酸钠(SDS)聚丙烯酰胺凝胶电泳,发现抗源蛋白得到有效降解,分解为相对分子质量小于10 000的肽类物质。
2.
This paper dealt with LDH isozymes of heart, l ung, kidney, stomach, skin, tongue, later limbs muscle, liver of amphibians in Rana nigromaculata Hallowell, Rana Limnocharis Boie,Microhyla oranata (Dumeril et Biorn),Bufo melanostictus Schneider and Rana catesbeiana shaw by using polyacrylamide gel electrophoresis.
采用聚丙烯酰胺凝胶电泳技术 ,研究了浙江金华五种蛙 :黑斑蛙、泽蛙、饰纹姬蛙、黑眶蟾蜍和牛蛙的心肌、肺、肾、胃、皮、舌、后肢肌肉和肝共 8种组织的乳酸脱氢酶 (LDH)同工酶 ,结果显示LDH具有明显的种族特异性和组织特异性 ,即不同动物和同种动物的不同组织都有各自的LDH同工酶谱型 。
5) gelelectrophoresis
凝胶电泳
1.
Results Apoptosisoccurredin themacrophagestreatedwithdexamethasone,andDNAfragmentationof theapoptoticmacrophageswasvisualizedon1%agarosegelelectrophoresis.
结果经地塞米松处理的巨噬细胞发生细胞凋亡,用1%琼脂糖凝胶电泳出现典型梯状条带,同时用流式细胞术检测出现凋亡峰,计算机凝胶图像光密度分析电泳结果,地塞米松处理0、0。
补充资料：Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳（sds-page，sodium dodecyl sulfate-polyacrylamide gel electrohoresis）
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的，而不是根据分子所带的电荷和大小分离的。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条