MethodsThe methods of agarose gel electrophoresis,microscope fluorescence of Hoechst 33342 fluorescence dye and flow cells analysis were used in the experiment.

Single cell gel electrophoresis is applied for genetic toxicology,DNA damage and detection,environmental pollution detection,apoptosis mechanism research et al.

In general, those problems such as the dim and bending band, strong fluorescence background, bad repetition often occured during DNA agarose gel electrophoresis.

The application of molecular methods such as hybridiaztion of nucleic acid probes, amplification of polymerase chain reaction (PCR), latest gel electrophoresis, quinone profiles and analyses of ribosomal RNA will extend the research field of microbial ecology, and make it possible to study the mechanism of ecology problem at molecular level.

Selenoproteins in soybean from Ensh area where selenium in soil was at a higher level were seperated by using sodium dodecyl sulfste-polyacrylamide gel electrophoresis (SDS-PAGE) and then identified by using high Performance liquid chromatography with a fluorescent indication detector (HPLC-FID).

SDS-PAGE results showed there were 4 types of protein zones.

UV and SDS-PAGE analysis suggest that the extraction product is typeⅠcollagen,and its molecule mass is about 320 kDa.

SDS electrophoresis confirmed the antigen protein was effectively degraded.

This paper dealt with LDH isozymes of heart, l ung, kidney, stomach, skin, tongue, later limbs muscle, liver of amphibians in Rana nigromaculata Hallowell, Rana Limnocharis Boie,Microhyla oranata (Dumeril et Biorn),Bufo melanostictus Schneider and Rana catesbeiana shaw by using polyacrylamide gel electrophoresis.

Results Apoptosisoccurredin themacrophagestreatedwithdexamethasone,andDNAfragmentationof theapoptoticmacrophageswasvisualizedon1%agarosegelelectrophoresis.