DEAD-box RNA helicases are involved in various aspects of RNA metabolism, including RNA transcription, premRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation and RNA decay.

The DEAD-box RNA helicases is a big ATP-dependent RNA helicases family, which originates from a wide range of organisms ranging from prokaryotes, including viruses, to eukaryotes such as yeast, plants and animals.

DEAD-box family proteins are a putative ATP-dependent RNA helicase, which form a widely dispersed family of proteins ranging from viruses to mammals, and are associated with nearly all RNA processes.

In this thesis, cDNAs of reproduction-related genes including Cf-vasa, Cf-PL10 and Cf-p68 of DEAD-box family and Cf-boule of DAZ f.

Molecular characterization of a rice DEAD-box RNA helicase gene OsBIRH1 and purification of recombinant protein;

Dengue virus NS3 protein is a multifunctional protein that has been shown to have protease, RNA helicase, NTPase and 5′-terminal RNA triphosphatase activities.

The P68 nuclear protein is an ATP-dependent RNA helicase a nd RNA-dependent ATPase, and has been proved to have functions in the regulati on o f cell growth and division.

The ATP dependent RNA helicases of the DEAD box family, involved in almost every cellular process of RNA metabolism, play many essential roles during cell development and growth in almost all organisms.