Partial ATPase/RNA helicase functional domain of NS3 genes of CSFV SHIMEN rain and C rain were amplified by RT-PCR;The recombinant plasmids pET-NS3 SMand p ET-NS3C were obtained after being cloned them into expression vector pET-28a(+);And then the 54 kD target proteins were produced by inducing with 0.

Dengue virus NS3 protein is a multifunctional protein that has been shown to have protease, RNA helicase, NTPase and 5′-terminal RNA triphosphatase activities.

The P68 nuclear protein is an ATP-dependent RNA helicase a nd RNA-dependent ATPase, and has been proved to have functions in the regulati on o f cell growth and division.

The ATP dependent RNA helicases of the DEAD box family, involved in almost every cellular process of RNA metabolism, play many essential roles during cell development and growth in almost all organisms.

DEAD-box RNA helicases are involved in various aspects of RNA metabolism, including RNA transcription, premRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation and RNA decay.

The DEAD-box RNA helicases is a big ATP-dependent RNA helicases family, which originates from a wide range of organisms ranging from prokaryotes, including viruses, to eukaryotes such as yeast, plants and animals.

Relationship between RNA helicases and tumor;