1) CD40Ig gene transfer
CD40Ig基因转染
2) gene transfection
基因转染
1.
Effects of FATE/BJ-HCC-2 gene transfection on biological behavior of tumor cells;
FATE/BJ-HCC-2基因转染对肿瘤细胞生物学行为的影响
2.
Effects of Canstatin gene transfection on growth and apotosis of HUV-ECC cells in vitro;
Canstatin基因转染对体外培养血管内皮细胞增殖与凋亡的影响
3.
Expression and biological effects of human BMP-2 gene transfectionon rabbit s bone mesenchymal stem cells in vitro;
hBMP-2基因转染兔骨髓间充质干细胞的表达及生物学效应的观察
3) Gene transfer
基因转染
1.
Protective immunity effect of Sj26 gene transfer dendritic cells on Schistosoma japonicum infection;
Sj26基因转染树突状细胞抗血吸虫感染作用
2.
NT-3 gene transfer to facial muscle denervation in rats;
大鼠失神经支配表情肌NT-3基因转染的实验研究
3.
IL-10 gene transfer prolongs the survival of allografts in rats;
白细胞介素10基因转染延长移植物存活的研究
4) Transfection
[英][træns'fekʃən] [美][træns'fɛkʃən]
基因转染
1.
Methods The plasmid of bacteriophague expression vector pBK-bax, which contains human bax cDNA, was transduced into HCC cell line HCC-9204 cells with Lipid-mediated gene transfection method, and at the same time the HCC-9204 cells transfected with empty pBK-CMV plasmid and non-transfected HCC-9204 cells were taken as control.
方法用脂质体介导的基因转染法将含有人bax cDNA的噬菌粒表达载体pBK-bax质粒导入人肝癌细胞系HCC-9204细胞中,同时设转染pBK-CMV空载体的和未转染的HCC-9204细胞对照。
2.
Objective: To observe the lethal effect of T cells activated by dendritic cells (DCs) with MUC1 gene transfection (MUC1-DCs) on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfected by MUC1 gene as a vaccine for bladder cancer immunotherapy.
方法应用脂质体将人MUC1基因转染体外培养的外周血来源的DCs,并活化T细胞,以流式细胞术和MTT法检测其转染率和免疫活性,以不同效靶细胞的比例对BIU-87细胞和膀胱正常上皮细胞进行体外杀伤实验,MTT法测定杀伤率。
3.
Nonetheless,transfection of LMO2 into Molt-4 cells increased plating efficiency.
方法荧光实时定量聚合酶链反应;Western印迹实验;基因转染建立高表达LMO2的白血病细胞株;siRNA干扰技术;细胞克隆形成实验。
5) gene transduction
基因转染
1.
Investigation of the effect of C2 gene transduction on human gastric cancer cell SGC7901;
C2基因转染对人胃癌细胞SGC7901的影响
2.
A recombinant adenovirus Adexl CAmILl0 enabled us to achieve very efficient gene transduction and hi.
鉴于C26结肠癌细胞转染了IL-10基因后在体内的致瘤性明显下降,本研究构建了一株对C26结肠癌细胞具有杀伤活性的CD8~+CTL并观察了IL-10基因转染的该细胞株对肿瘤的治疗作用。
6) gene transfect
基因转染
1.
Influence of human antisense-VEGF gene transfection to Hep-2 cells in vitro and in vivo;
反义VEGF基因转染对HEP-2细胞生物学行为的影响
2.
Objective To study endothelial nitric oxide synthase (eNOS) gene transfecting endothelial cells (ECs).
结论通过脂质体法成功地将eNOS基因转染于实验犬EC;转染后eNOS基因在mRNA和蛋白质水平均高效表达;内皮细胞eNOS活性显著增强;转染后细胞生物学功能稳定。
3.
Objective:To investigate the effect of Fas gene transfection on tumorigenicity and proliferation of transplanted tumor of human buccal squamous cell carcinoma cell line BcaCD 885 cell.
结论 :Fas基因转染抑制移植瘤形成和增殖与提高Fas表达、增加肿瘤细胞凋亡有关。
补充资料：转染
转染 （transfection）采用与质粒dna转化受体细胞相似的方法，即宿主菌先经过cacl2，电穿孔等处理成感受态细菌，再将重组?噬菌体dna直接导入受体细胞，进入感受态细菌的噬菌体dna可以同样复制和繁殖，这种方式称为转染。
转染是转化的一种特殊形式。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条