1) Akt gene transfection
Akt基因转染
1.
Objective To elucidate the effects of Akt gene transfection on apoptosis,proliferation and glucose uptake of bone marrow mesenchymal stem cells(MSCs) of Wistar rats,under hypoxia.
目的采用Akt基因转染鼠骨髓MSCs探讨Akt基因是否减轻MSCs缺氧时的凋亡和提高缺氧时的增殖能力,即耐缺氧能力。
2) genes Akt
基因Akt
3) Akt gene
Akt基因
1.
Effect of transgenic human Akt gene ion apoptosis of cardiac myocyte after ischemia-reperfusion injury
阳离子脂质体转染Akt基因对缺血性心脏病细胞凋亡的影响
4) gene transfection
基因转染
1.
Effects of FATE/BJ-HCC-2 gene transfection on biological behavior of tumor cells;
FATE/BJ-HCC-2基因转染对肿瘤细胞生物学行为的影响
2.
Effects of Canstatin gene transfection on growth and apotosis of HUV-ECC cells in vitro;
Canstatin基因转染对体外培养血管内皮细胞增殖与凋亡的影响
3.
Expression and biological effects of human BMP-2 gene transfectionon rabbit s bone mesenchymal stem cells in vitro;
hBMP-2基因转染兔骨髓间充质干细胞的表达及生物学效应的观察
5) Gene transfer
基因转染
1.
Protective immunity effect of Sj26 gene transfer dendritic cells on Schistosoma japonicum infection;
Sj26基因转染树突状细胞抗血吸虫感染作用
2.
NT-3 gene transfer to facial muscle denervation in rats;
大鼠失神经支配表情肌NT-3基因转染的实验研究
3.
IL-10 gene transfer prolongs the survival of allografts in rats;
白细胞介素10基因转染延长移植物存活的研究
6) Transfection
[英][træns'fekʃən] [美][træns'fɛkʃən]
基因转染
1.
Methods The plasmid of bacteriophague expression vector pBK-bax, which contains human bax cDNA, was transduced into HCC cell line HCC-9204 cells with Lipid-mediated gene transfection method, and at the same time the HCC-9204 cells transfected with empty pBK-CMV plasmid and non-transfected HCC-9204 cells were taken as control.
方法用脂质体介导的基因转染法将含有人bax cDNA的噬菌粒表达载体pBK-bax质粒导入人肝癌细胞系HCC-9204细胞中,同时设转染pBK-CMV空载体的和未转染的HCC-9204细胞对照。
2.
Objective: To observe the lethal effect of T cells activated by dendritic cells (DCs) with MUC1 gene transfection (MUC1-DCs) on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfected by MUC1 gene as a vaccine for bladder cancer immunotherapy.
方法应用脂质体将人MUC1基因转染体外培养的外周血来源的DCs,并活化T细胞,以流式细胞术和MTT法检测其转染率和免疫活性,以不同效靶细胞的比例对BIU-87细胞和膀胱正常上皮细胞进行体外杀伤实验,MTT法测定杀伤率。
3.
Nonetheless,transfection of LMO2 into Molt-4 cells increased plating efficiency.
方法荧光实时定量聚合酶链反应;Western印迹实验;基因转染建立高表达LMO2的白血病细胞株;siRNA干扰技术;细胞克隆形成实验。
补充资料:转染
转染 (transfection)采用与质粒dna转化受体细胞相似的方法,即宿主菌先经过cacl2,电穿孔等处理成感受态细菌,再将重组?噬菌体dna直接导入受体细胞,进入感受态细菌的噬菌体dna可以同样复制和繁殖,这种方式称为转染。
转染是转化的一种特殊形式。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条