2) cloning of light and heavy chain genes
抗体基因克隆
3) cloning vector
克隆载体
1.
Construction of cloning vector of c-myc-TFF2 fusion gene by PCR based gene assembly;
基因拼装构建c-myc-hTFF2融合基因克隆载体pBS-TFF2
2.
The required fragments were selected with correspording restriction endonuclease and inserted into suitable cloning vector by two steps.
用碱裂解法抽提质粒后，利用相应的限制性内切酶切出目的基因片段，连接到合适的载体上，构建了完整的ＭＤＶＢ抗原基因克隆载体。
3.
A protruding T on 3blunt end of the cloning vector was created by adding dTTP and Taq DNA polymerase to the reaction mixtures.
将克隆载体平端切割后，向反应体系中加入ｄＴＴＰ，用ＴａｑＤＮＡ聚合酶处理载体，使其３－端带上一个凸出的单碱基Ｔ，修饰后的载体可直接与纯化的ＰＣＲ产物连接。
4) UK114 gene cloning and construction of expression vector
UK114基因克隆及表达载体构建
5) gene clone
基因克隆
1.
Study and development on isolation,purification, gene clone and functions of lectins;
凝集素的分离纯化、基因克隆及功能研究进展
2.
Gene Clone and HighLevel Expression of Staphylokinase;
葡萄球菌激酶(Sak)的基因克隆和高效表达工程菌的构建
3.
To explore the effect of a tyrosine kinase inhibitor STI571 and P21 WAF gene clone on the proliferation, cycle, apoptosis of leukemia cell line K562, P21 WAF gene was obtained by RT PCR, and its sequence was approved to be correct, then P21 pcDNA3.
本研究探讨酪氨酸激酶抑制剂STI5 71和P2 1WAF基因克隆对慢性粒细胞白血病急变K5 62细胞株的细胞增殖、细胞周期及凋亡等的治疗作用。
6) gene cloning
基因克隆
1.
Study on gene cloning of Chlamydial pneumonia CPn0308 and its endogenous localization;
肺炎衣原体CPn0308的基因克隆及其内源性蛋白定位的研究
2.
Gene cloning of glycoprotein of Hantavirus and construction of G1 and G2 glycoprotein baculovirus expression vectors;
汉坦病毒G1和G2糖蛋白的基因克隆及糖蛋白杆状病毒表达载体的构建
3.
Gene cloning, expression and purification of Shiga like toxin 2B from EHEC O157∶H7;
EHECO157∶H7志贺样毒素Ⅱ结合亚单位Stx2B的基因克隆、表达与纯化
补充资料：植物克隆载体
分子式：
CAS号：
性质：植物基因操作中之携带外来DNA的载体，并以可遗传的方式将它嵌入植物细胞之内。天然的载体有根癌根杆菌(Agrobacterium tumefaciens)的Ti质粒或发根农杆菌(A. rhizogenes)的Ri质粒以及DNA植物病毒。现在因Ti或Ri质粒太大，不易操作，常取其DNA复制及引发癌肿的基因与普通细菌质粒组成穿梭质粒，便于先在细菌(尤其是大肠杆菌)中操作、扩增，然后再引入植物。
CAS号：
性质：植物基因操作中之携带外来DNA的载体，并以可遗传的方式将它嵌入植物细胞之内。天然的载体有根癌根杆菌(Agrobacterium tumefaciens)的Ti质粒或发根农杆菌(A. rhizogenes)的Ri质粒以及DNA植物病毒。现在因Ti或Ri质粒太大，不易操作，常取其DNA复制及引发癌肿的基因与普通细菌质粒组成穿梭质粒，便于先在细菌(尤其是大肠杆菌)中操作、扩增，然后再引入植物。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条