1) In vitro transcription and translation
体外转录翻译
2) transcription and translation
转录翻译
3) replication and experssion
转录与翻译
4) in vitro translation
体外翻译
1.
A homologous chloroplast in vitro translation system of chloroplast encoded proteins D_1, cytochrome f (Cytf), cytochrome b_6 (Cytb_6) was established to systematically study the molecular mechanism of chloroplast encoded proteins assembly and regulatory processes.
以高等植物豌豆和烟草叶绿体基因编码的蛋白D1,细胞色素f和细胞色素b_6为研究对象,采用分子生物学基因克隆,体外转录,蛋白质放射自显影分析技术,构建具有翻译活性的同源叶绿体体外翻译系统,以系统深入地研究叶绿体基因编码蛋白的类囊体膜插入合成组装调控的分子机制。
5) in vitro transcription
体外转录
1.
In vitro transcription of eIF4E in laryngeal carcinoma;
喉癌eIF4E基因的体外转录
2.
Methods Transcription initiation at promoters of five genes selected from the two groups based on the transcriptome analysis was studied by means of in vitro transcription system.
目的研究转录调控蛋白PrfA对两组新近发现的单核细胞增生李斯特菌基因的体外转录作用。
3.
Objective To synthesize highly pure EBV-LMP1 carboxy region RNA by in vitro transcription and select its targeted active DNAzyme.
目的探讨用体外转录方法制备EBV-LMP1C端RNA并用该RNA对其靶脱氧核酶进行初步筛选。
6) transcription in vitro
体外转录
1.
Digoxigenin(DIG) labeled RNA probes were prepared by transcription in vitro in order to establish ISH(in situ hybridization) method.
根据hsp70和hsp90 mRNA基因序列,设计并合成引物,将PCR扩增的基因片段克隆到pGEM-T载体,重组质粒经筛选、鉴定,利用体外转录系统,制作地高辛标记的RNA探针,建立检测hsps mRNA原位杂交方法。
2.
4 kb) which is complementary to both coding region and 5 terminus franking region (upstream the initiator codon AUG) have been obtained by transcription in vitro with SP6 RNA polymerase or T7 RNA polymerase from the plasmids, pSP72-C12 and pSP72-C1.
体外转录载体ｐＳＰ７２—Ｃ１２和ｐＳＰ７２—Ｃ１。
3.
The Study of Amplifing HCV ssRNA by RT-PCR Combined With Transcription in vitro;
本研究旨在以HCV为平台,在简化RT-PCR基础上,结合体外转录,建立一种特异、高效、简便的检测血清中HCVRNA的体外转录合成系统。
补充资料：体外转录
分子式：
CAS号：
性质：在试管中对一个结构基因5′一端加上启动子，3′端加终止子在四种核苷三磷酸存在下经RNA聚合酶作用即可以结构基因为样板进行转录，合成RNA。
CAS号：
性质：在试管中对一个结构基因5′一端加上启动子，3′端加终止子在四种核苷三磷酸存在下经RNA聚合酶作用即可以结构基因为样板进行转录，合成RNA。
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参考词条