1) chromosome C-banding/fluorescence in situ hybridization (FISH)
C-分带/原位杂交
2) Sequent C-banding-GISH
连续C-分带-基因组原位杂交
3) In situ hybridization
原位分子杂交
1.
Methods In situ hybridization,immunofluorescence double staining and Western blotting methods were used.
方法原位分子杂交、免疫荧光双重染色和Western blotting。
2.
Reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization were used to examine the expression levels of INSL3 mRNA in primarily cultured Leydig cells incubated with.
方法:DEHP50,100,200mg/L分别作用于原代培养的小鼠胚胎Leydig细胞,应用RT-PCR和原位分子杂交技术检测DEHP对Leydig细胞INSL3mRNA表达的影响;DEHP分别以低、中、高三组剂量(100,200和500mg/kg·d)灌胃作用于GD(Gestationday)12~PND(Postnatalday)3孕鼠,RT-PCR检测新生小鼠睾丸INSL3mRNA表达的相对强度。
3.
The expression of cyclinD1,cyclinE and CDK6mRNA in hepatocellular carcinoma tissues,para-carcinoma liver tissues(twenty cases,respectively) and normal liver tissues(five cases) were detected by immunohistochemistry,in situ hybridization and cell image analysis.
应用免疫组织化学、原位分子杂交和细胞图象分析技术检测原发性肝细胞癌组织及其对应的癌旁肝组织(各20例)、正常肝组织(5例)中CyclinD1、CyclinE和CDK6mRNA表达情况。
4) in situ hybridization
分子原位杂交
1.
Identification of human papillomaviruses in breast cancer by electron microscopy and DNA in situ hybridization;
人乳腺癌HPV感染的超微结构和DNA分子原位杂交研究
2.
Analysis of the Chromosomes Composition of Common Wheat-Agropyronintermedium Partial Amphiploid, Yuan Zhong 2 by in situ hybridization;
应用分子原位杂交技术解析小麦-天兰冰草部分双二倍体──远中2号的染色体构成
3.
In situ hybridization by using biotin labelled genomic DNA of H.
用生物素标记的簇毛麦（Ｈａｙｎａｌｄｉａｖｉｌｌｏｓａ）染色体组ＤＮＡ（ｔｏｔａｌｇｅｎｏｍｉｃＤＮＡ）作探针，以普通小麦染色体组ＤＮＡ作遮盖（用量１：２００左右），进行有丝分裂中期和减数分裂中期Ｉ染色体的分子原位杂交（ＧＩＳＨ），经抗生物素蛋白－辣根过氧化物酶复合物（ｂｉｏ－ｓｔｒｅｐｔａｖｉｄｉｎ－ｈｏｒｓｅｒａｄｉｓｈｐｅｒｏｘｉｄａｓｅ）和联苯胺四盐酸（ＤＡＢ）检测显色后，小麦－簇毛麦双倍体、附加系、代换系和易位系中的簇毛麦染色体及染色体片段显棕色，与显浅蓝色的小麦染色体可明显区分。
5) in situ hybridization
原位杂交
1.
Expression of Pax3 gene in Chick Embryo spinal cord analyzed with the improved in situ hybridization;
应用改良的原位杂交方法分析Pax3基因在鸡胚脊髓中的表达
2.
Complex chromosomal aberrations detected by fluorescence in situ hybridization and their clinical significance in patients with myelodysplastic syndrome;
荧光原位杂交检测骨髓增生异常综合征患者染色体复杂核型变化及临床意义的研究
3.
Establishing of in situ hybridization method for the expression of dentin phosphoprotein mRNA;
牙本质磷蛋白mRNA原位杂交方法的建立
6) in-situ hybridization
原位杂交
1.
A whole-mount in-situ hybridization study on expression profile of mCcd1 during mouse embryonic development;
整体原位杂交研究mCcd1在小鼠胚胎发育过程中的表达
2.
Methods: In-situ hybridization and immunohistochemistry were used to detect the expressions of CyclinE mRNA and CyclinE protein in 40 cases of colon carcinoma in various stages,and 10 colon adenoma,and 10 normal colon mucosa.
方法:采用原位杂交方法和免疫组化S-P法对40例结肠癌1、0例结肠腺瘤和10例结肠正常粘膜组织进行CyclinE mRNA和CyclinE蛋白的检测,同时结合临床病理资料进行分析。
3.
The hSAMP32 mRNA expression was detected by in-situ hybridization.
原位杂交检测hSAMP32mRNA在3组细胞中的表达。
补充资料：荧光原位杂交
荧光原位杂交
是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针，依据碱基配对原理，通过免疫细胞化学检测体系，在组织切片、细胞间期核或染色体等标本上，进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高，特异性强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条