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1)  CHO dhfr-cells
CHO dhfr~-细胞
2)  CHO/dhfr~-cell
CHO/dhfr-细胞
1.
AIM: To obtain CHO/dhfr~- cells line with integrated FRT sequence in the chromosome transcription active site and to express human-mouse chimeric antibody directed against Chikungunya Virus by using the cell line.
以此载体转染CHO/dhfr-细胞,用1g/L的G418筛选抗性克隆。
3)  CHO-dhfr-
CHO-dhfr细胞
4)  CHO dhfr cell
CHO-dhfr~-细胞
5)  CHO-dhfr-cell
CHO-dhfr-细胞
1.
After tranfection with CHO-dhfr- cell line, fibrinolysis activity was detected in the cultivate supernatant.
利用携带有二氢叶酸还原酶(dhfr)基因的pCI载体,实现tPA突变体(FrGGI)在CHO-dhfr-细胞中的高效表达,获得高表达细胞株。
2.
AIM: To obtain high level expression of recombinant human truncated osteoprotegerin(TOPG) with higher bioactivity in CHO-DHFR-cells.
1/DHFR-hTOPG,实现其在CHO-DHFR-细胞中的高效表达,获取生物活性较高的重组蛋白。
6)  CHO/dhfr
CHO/dhfr-
1.
Results: The bioinformatic analysis indicated BQ135232 possessed the possibility to be an immunogen, subsequently we constructed the expressing vector pcDNA3-BQ-ORF-GPI, transfected CHO/dhfr-successfully, and verified by RT-PCR.
方法:应用生物信息学方法分析BQ135232 mRNA基因序列,初步了解其基本性状,构建表达载体,转染真核细胞(CHO/dhfr-)。
补充资料:CHO细胞
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性质:一个转化细胞系,1957年从中国地鼠卵巢细胞得到。广泛采用的K1亚克隆株需要脯氨酸,因为遗传学上有缺陷,不能将谷氨酸转变为谷氨酸-γ-半醛。该细胞是上皮样细胞,通常贴壁生长,也可悬浮生长。CHO细胞被广泛地用来表达重组DNA的蛋白。

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