1) CHO-K1 cell
CHO-K1细胞
1.
Methods CHO-K1 cells were transfected by pSNAV-signal-syn BPI_(m600)-Fcγ1_(700) expression vector,positive cell clones which high-expressed BPI_(m23)-Fcγ1 were selected with Dot blot and cultured in PF-CHO medium to express functional BPI_(m23)-Fcγ1 recombinant protein.
方法用pSNAV-signal-syn BPIm600-Fcγ1700转染CHO-K1细胞,通过Dot blot筛选获得高表达BPIm23-Fcγ1目的蛋白的阳性细胞克隆,经PF-CHO培养基扩大培养,获得分泌表达的BPIm23-Fcγ1重组抗菌蛋白。
2.
pIRES-N was transfected into CHO-K1 cells with coprecipitation, and positive cells were screened with hygromycine selection.
然后通过磷酸钙共沉淀法转染CHO-K1细胞,通过潮霉素筛选得到阳性克隆,间接免疫荧光实验(IFA)鉴定N基因在CHO细胞中的表达,并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达,最终建立了CHO/CDV-N细胞株,为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。
3.
Objective:To optimize the condition of gene transient transfection in CHO-K1 cell.
目的:以CHO-K1细胞为宿主基因瞬时转染条件的优化。
2) CHO-K1 cells
CHO-K1细胞
1.
Expression of porcine Toll-like receptor TLR2 gene in the CHO-K1 cells;
猪TOLL样受体2基因在CHO-K1细胞中的表达
2.
And finally,pcDNA3/hLIF was introduced into COS-7 cells and CHO-K1 cells by using the method of lipofectin transfection,and transient expression and stable expression of hLIF protein was obtained respectively.
利用脂质体介导将这一表达载体导入COS-7细胞和CHO-K1细胞,分别获得了hLIF的瞬时表达和稳定表达,为进一步进行hLIF生物学功能研究和hLIF在哺乳动物细胞中的高表达研究奠定了基础。
3) CHO-K 1cell line
CHO-K1细胞系
4) CHO cell
CHO细胞
1.
Construction of eukaryotic expression vector of HBsAg and its expression in CHO cell;
HBsAg真核表达载体的构建及其在CHO细胞中的表达
2.
Establishment and identification of the CHO cell lines which express attenuated mutants of Shiga-like toxin Ⅰ;
稳定表达突变减毒志贺样毒素Ⅰ的CHO细胞株的建立与鉴定
3.
Determination of hOP-1 full-length gene expression in CHO cells and biological activity of rhOP-1;
hOP-1全长基因在CHO细胞的表达及活性检测
5) CHO cells
CHO细胞
1.
Construction of decorin expression vector and its expression in CHO cells;
核心蛋白聚糖真核表达载体的构建及其在CHO细胞中的表达
2.
Effect of wild-type and mutant DNA polymerase β expression on CHO cells growth;
不同类型的DNA聚合酶β基因对CHO细胞的影响
3.
Expression of murine bcl-X_L gene in CHO cells;
鼠bcl-X_L基因在CHO细胞中的表达
6) CHO/dhfr~-cell
CHO/dhfr-细胞
1.
AIM: To obtain CHO/dhfr~- cells line with integrated FRT sequence in the chromosome transcription active site and to express human-mouse chimeric antibody directed against Chikungunya Virus by using the cell line.
以此载体转染CHO/dhfr-细胞,用1g/L的G418筛选抗性克隆。
补充资料：CHO细胞
分子式：
CAS号：
性质：一个转化细胞系，1957年从中国地鼠卵巢细胞得到。广泛采用的K1亚克隆株需要脯氨酸，因为遗传学上有缺陷，不能将谷氨酸转变为谷氨酸-γ-半醛。该细胞是上皮样细胞，通常贴壁生长，也可悬浮生长。CHO细胞被广泛地用来表达重组DNA的蛋白。
CAS号：
性质：一个转化细胞系，1957年从中国地鼠卵巢细胞得到。广泛采用的K1亚克隆株需要脯氨酸，因为遗传学上有缺陷，不能将谷氨酸转变为谷氨酸-γ-半醛。该细胞是上皮样细胞，通常贴壁生长，也可悬浮生长。CHO细胞被广泛地用来表达重组DNA的蛋白。
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参考词条