Four methanogenic granules taken from an anaerobic reactor in different periods were investigated by FISH and DGGE,the eubacterial and archaeal community in these granules was researched and the phylogenetic analysis of dominant archaea was also studied.

Effect of ammonium concentration on the methanogenic activity of anaerobic granular sludge;

Batch experiments were conducted to study the effect of low concentration of Ce~(3+)on the activity of anaerobic granular sludge in the state of start-up,stabilization,acidification and restart-up by determining its specific methanogenic activity(SMA),extra cellar polysaccharides and nucleic acids content.

Based on biochemical methane potential (BMP) and toxicity assay on anaerobic biomass at different concentration of polyquaternary amine, an appraisal was conducted on anaerobic biodegradability of polyquaternary amine, and comparison was made on the effect of various polyquaternary amine concentration on specific methanogenic activity (SMA) and settleability (SVI) of anaerobic biomass.

Effect of floccule addition on specific methanogenic activity of anaerobic microbe;

In the experiement,reactive methane bacteria immobilized carrier made by investing method with anaerobic methane flora and conventional methane bacteria sludge are put into the 20-litre glass anaerobic jars to form methane bacteria immobilized carrier system and methane baeteria system respectively.