Cloning and sequencing of PGIP cDNA from Prunus mahaleb;

The PGIP gene in Prunus mume Sieb et Zucc.

A target fragment with a full length of 1 192 bp(Genbank accession:DQ200847) was amplified with genomic DNA of Prunus salicina leaves as the templates and the conservative sequences of PGIP gene as the primers.

By inserting PGIP in vector P-Super1300+ with super-promoter and nos terminator,a plant super-expressed vector of PGIP gene from Prunus mume was constructed successfully and then transferred to tobacco by Agrobacterium-mediated transformation system.

By using the low - ion buffer and high - ion buffer, the soluble and insoluble PGIP were obtained through ammonium sulfate precipitation, CM - Sepharose FF and Sephadex G - 100 chromatography.

By using the low - ion buffer and high - ion buffer, the soluble and insoluble PGIP were obtained through ammonium sulfate precipitation, CM - Sepharose FF and Sephadex G - 100 chromatography.

Partial structure of wheat PGIP was studied in four aspect: deglycosylation, analysis of partial primary structure, secondary structure and disulfide bond.

Cloning and Sequence Analysis of PGIP gene and cDNA from Prunus persica

Polygalacturonase-inhibiting proteins(PGIPs) belong to a kind of protein family which have the structure of Leucine-rich repeat(LRR) and are primarily localized in the cell wall.