1) 5-upsteam promoter region single-specific-primer PCR
5'上游区单一特异引物聚合酶链反应
2) PCR-SSP
聚合酶链反应-序列特异性引物
1.
Methods A total of 192 RhD positive samples,and 166 RhD negative samples were tested by PCR-SSP for exons of RHC/E and D genes.
方法用聚合酶链反应-序列特异性引物(PCR-SSP)扩增技术,扩增Rh血型C/E基因,D基因的外显子,检测192例RhD阳性个体,166例RhD阴性个体的血样品。
2.
Methods:HLA-DRB alleles of 82 vitiligo patients and 80 psoriasis vulgaris patients were detected by polymerase chain reaction sequence-specific primer(PCR-SSP),under the comparison with 86 healthy persons.
方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)分型技术,对82例汉族白癜风患者和80例寻常型银屑病患者HLA-DRB1等位基因进行检测,与86名汉族健康人群进行对照。
3.
Methods A total of 88 umbilical cord blood samples,including those randomly selected from Tianjin stem cell bank and already matched for transplantation,were performed polymerase chain reaction sequence specific primers(PCR-SSP) and sequencing based type (SBT) to check their type results and sequencing based type.
方法采用聚合酶链反应-序列特异性引物扩增(PCR-SSP)方法、直接测序分型穴SBT雪方法,随机抽查天津市脐带血造血干细胞库脐带血及查询已选中做脐带血移植的样本共88例SSOP方法的结果进行HLA基因分型复核。
3) Polymerase chain reaction with sequence specific primers
引物特异性聚合酶链反应
4) polymerase chain reaction-sequence specific primer
聚合酶链反应-序列特异性引物
1.
Methods HLA-DR15 was detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) and compared between peripheral blood of MDS group(n=59)and control group(n=104).
方法采用聚合酶链反应-序列特异性引物(PCR-SSP)检测MDS患者(n=59)HLA-DR15基因的表达,并与健康对照组(n=104)进行比较。
2.
Methods Polymerase chain reaction-sequence specific primer (PCR-SSP) was used to analyze the distribution of HLA-A alleles among 106 patients with systemic lupus erythematosus and 122 healthy persons.
方法应用聚合酶链反应-序列特异性引物(PCR-SSP)的方法,对106例SLE患者和122例健康人进行HLA-A等位基因的检测。
5) PCR-SSP
聚合酶链反应-序列特异引物
1.
Methods: The HLA-DRB1 alleles were detected by a PCR-SSP technique in 34 patients with generalized vitiligo and 262 normal controls in northern China.
方法:采用聚合酶链反应-序列特异引物(PCR-SSP)技术检测34例北方汉族泛发型白癜风患者的HLA-DRB1等位基因。
2.
Methods: With high-resolution polymerase chain reaction amplification with sequence specific primers(PCR-SSP),we detected the distribution frequencies of HLA-DRB1*07 allele among 60 cases with pv and 80 healthy controls and compared.
方法采用聚合酶链反应-序列特异引物(PCR-SSP)法,检测60例寻常型银屑病患者及80名健康对照者的等位基因频率,并相互比较。
3.
Methods Using method of high-resolution polymerase chain reaction amplification with sequence specific primers (PCR-SSP),we detected the distribution frequencies of HLA-DRB1*0701 allele among 42 patients with psoriasis vulgaris,28 patients with psoriasis pustulosa and 50 healthy patients as the control,and they were compared.
方法采用聚合酶链反应-序列特异引物(PCR-SSP)法检测42例寻常性银屑病、28例脓疱性银屑病和50例健康对照者HLA-DRB1*0701等位基因频率,并相互比较。
6) Polymerase chain reaction-sequence specific primers
聚合酶链反应/序列特异性引物
补充资料:链反应
链反应 chain reaction 通过在反应过程中交替和重复产生的活性中间体(自由基或自由原子)而使反应持续进行的一类化学反应。如反应H2+Cl2→2HCl的机理如下: Cl2+M→2Cl+M(1) Cl+H2→HCl+H(2) H+Cl2→HCl+Cl (3) …………… 2Cl+M→Cl2+M(4) 在反应(1)中,靠热、光、电或化学作用产生活性组分——氯原子,随之在反应(2)、(3)中活性组分与反应物分子作用而交替重复产生新的活性组分——氯原子和氢原子,使反应能持续不断地循环进行下去,直到活性组分消失,此即链反应。反应中的活性组分称为链载体。 链反应的机理一般包括3个步骤:①链引发。是依靠热、光、电、化学等作用在反应系统中产生第一个链载体的反应,一般为稳定分子分解为自由基的反应,如反应(1)。②链的传递。由链载体与饱和分子作用产生新的链载体和新的饱和分子的反应,如反应(2)、(3)。③链终止。链载体的消亡过程,如反应(4)。式中M为接受链终止所释放出能量的第三体(其他分子或反应器壁等)。 在链传递阶段,若一个旧的链载体消失只导致产生一个新的链载体,称为直链反应;若一个旧的链载体消失而导致产生两个或两个以上的新的链载体,则称为支链反应。 |
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