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1)  Sequence specific primer based polymerase chain reaction
序列特异性引物聚合酶链反应
1.
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction,in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因。
2)  PCR-SSP
序列特异性引物聚合酶链反应
1.
Materials and MethodsThe polymerase chain reaction-sequence specific primer(PCR-SSP) method was conducted in 158 patients with alopecia areata and 172 healthy controls in Eastern Han Chineses.
材料与方法采用序列特异性引物聚合酶链反应(sequence specific primers polymerasechain reaction,PCR-SSP)技术对已确诊为斑秃的158例患者和正常对照组172例进行HLA-DRB1基因多态性分析。
3)  PCR-SSP
聚合酶链反应-序列特异性引物
1.
Methods A total of 192 RhD positive samples,and 166 RhD negative samples were tested by PCR-SSP for exons of RHC/E and D genes.
方法用聚合酶链反应-序列特异性引物(PCR-SSP)扩增技术,扩增Rh血型C/E基因,D基因的外显子,检测192例RhD阳性个体,166例RhD阴性个体的血样品。
2.
Methods:HLA-DRB alleles of 82 vitiligo patients and 80 psoriasis vulgaris patients were detected by polymerase chain reaction sequence-specific primer(PCR-SSP),under the comparison with 86 healthy persons.
方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)分型技术,对82例汉族白癜风患者和80例寻常型银屑病患者HLA-DRB1等位基因进行检测,与86名汉族健康人群进行对照。
3.
Methods A total of 88 umbilical cord blood samples,including those randomly selected from Tianjin stem cell bank and already matched for transplantation,were performed polymerase chain reaction sequence specific primers(PCR-SSP) and sequencing based type (SBT) to check their type results and sequencing based type.
方法采用聚合酶链反应-序列特异性引物扩增(PCR-SSP)方法、直接测序分型穴SBT雪方法,随机抽查天津市脐带血造血干细胞库脐带血及查询已选中做脐带血移植的样本共88例SSOP方法的结果进行HLA基因分型复核。
4)  polymerase chain reaction-sequence specific primer
聚合酶链反应-序列特异性引物
1.
Methods HLA-DR15 was detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) and compared between peripheral blood of MDS group(n=59)and control group(n=104).
方法采用聚合酶链反应-序列特异性引物(PCR-SSP)检测MDS患者(n=59)HLA-DR15基因的表达,并与健康对照组(n=104)进行比较。
2.
Methods Polymerase chain reaction-sequence specific primer (PCR-SSP) was used to analyze the distribution of HLA-A alleles among 106 patients with systemic lupus erythematosus and 122 healthy persons.
方法应用聚合酶链反应-序列特异性引物(PCR-SSP)的方法,对106例SLE患者和122例健康人进行HLA-A等位基因的检测。
5)  Polymerase chain reaction-sequence specific primers
聚合酶链反应/序列特异性引物
6)  polymerase chain reaction-sequence specific primers
聚合酶链反应-序列特异性引物
1.
Methods The technique of polymerase chain reaction-sequence specific primers (PCR-SSP) was adopted in genotyping a sample of 8962 unrelated healthy individuals collected from a Chinese Han population in Liaoning area.
方法用聚合酶链反应-序列特异性引物方法对辽宁8962名健康无关汉族人进行HLA-B等位基因分型,计算HLA-B等位基因频率并与不同人群HLA-B等位基因的多态性进行比较。
补充资料：精子特异性酶乳酸脱氢酶X(LDHX)的测定


精子特异性酶乳酸脱氢酶X(LDHX)的测定


　　诊法。精液生化检查项目之一。测定方法主要用聚丙烯酰胺凝胶电泳，酶联染色及光密度计扫描法，可见6条区带，在LDH3与LDH4之间有一条LDHx区带，与生精能力有关。为精子细胞、精子和伴有精子的精液所有，与精子数量和酶的活性有关。可作为估价男子生育能力的客观指标。棉酚可特异性地抑制之，以影响生育。
　　
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