1)  Suppression subtractive hybridization (SSH)
差减抑制杂交
1.
Suppression subtractive hybridization (SSH) has been widely used in medical research but less in plant science up to date.
差减抑制杂交技术在医学研究中应用较多,而在植物研究中的应用较少,近几年有所增加。
2)  subtraction method
差减法
1.
The last step is that calculate the contents of SiO2 by subtraction method.
石英砂是玻璃厂主要的原材料,其主要成分SiO2的测定采用HF-H2SO4处理,使硅逸去,杂质成分在800℃灼烧称重,差减法计算SiO2的含量。
2.
The last step was calculating the content of SiO2 by subtraction method.
测定石英砂中SiO2的含量时,通常先用HF-H2SO4处理,使硅逸去,然后在800℃下灼烧、称重,利用差减法计算。
3)  differential chromatography
差减色谱
4)  spectrum subtraction
光谱差减
1.
To realize multi-component analysis of air quality detecting,an gas quantitative method combining technique of Fourier Transform Infrared (FTIR) spectroscopy and the method of spectrum subtraction was applied.
针对大气质量检测的多组分定量分析,本文提出利用傅里叶变换红外光谱(FTIR)技术与光谱差减相结合的气体定量方法。
5)  subtraction biopanning
差减筛选
1.
Methods Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library.
方法以骨肉瘤细胞os-732为靶细胞,成骨细胞为吸附细胞对噬菌体12肽库进行差减筛选,用细胞ELISA、免疫组化鉴定阳性噬菌体克隆并测序。
2.
Methods With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immuno.
方法以大肠癌LoVo细胞株作为靶细胞,人正常结肠黏膜上皮细胞为吸附细胞对噬菌体展示环七肽库进行差减筛选,采用细胞ELISA与免疫荧光检测鉴定噬菌体阳性克隆并测序。
6)  error reducing
误差减小
参考词条
补充资料：抑制杂交翻译
分子式：
CAS号：

性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。