说明:双击或选中下面任意单词,将显示该词的音标、读音、翻译等;选中中文或多个词,将显示翻译。
您的位置:首页 -> 词典 -> 差减杂交
1)  Subtractive hybridization
差减杂交
1.
Here we use the method of subtractive hybridization to i.
方法:本文的研究是基于心肌肥厚过程中各种基因在表达上所发生的变化,选用腹主动脉绑扎的左心室肥厚大鼠为模型,运用差减杂交法,旨在分离心肌肥厚过程中的相关基因。
2.
In the library,588 novel expressed sequence tags(ESTs) were obtained through suppression subtractive hybridization(SSH),and most of the identified ESTs were reported for the first time in amphioxus.
应用抑制性差减杂交(SSH)技术,通过对差减文库克隆序列的测定,共获得588个表达序列标签(EST)。
2)  suppression subtractive hybridization
抑制性差减杂交
1.
Suppression subtractive hybridization(SSHs) was performed to distinguish the differentially expressed genes.
取培养48h的2组细胞提取总RNA后分离mRNA,应用抑制性差减杂交法分离差异表达基因,并连接于pGEM TEasyVector,转化大肠杆菌JM109,构成差减cDNA文库,经蓝白斑筛选后挑选白色菌落,提取质粒,序列测定后进行同源性比较分析。
2.
A systematic study was initiated to identify differentially expressed genes in the process of sex reversal of the grouper by using suppression subtractive hybridization (SSH) technique.
应用抑制性差减杂交 (SSH)技术构建了石斑鱼性反转前后性腺组织的SMARTcDNA文库及其cDNA差减文库 ,从中随机挑取 12 0 0个克隆进行了PCR和斑点杂交筛选 ,得到 12 0个差异表达cDNA片段 。
3.
Objective: To Construct a subtracted cDNA library of hyperlipidemia sensitive rabbits′liver by suppression subtractive hybridization(SSH).
结论:用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。
3)  SSH
抑制差减杂交
1.
Isolation of Genes with Enhanced Expressions in Rice Treated with Probenazole Using SSH Technique;
用抑制差减杂交技术分离烯丙异噻唑诱导水稻特异表达的基因
2.
Principle and manipulation result analysis for suppression subtractive hybridization(SSH);
抑制差减杂交技术原理及常见操作结果分析
3.
To understand the gene expression characteristics,which involved in drought tolerance,five cDNA libraries were constructed using suppression subtractive hybridization(SSH) method with wheat seedlings sampled at 1,6,12,24 and 48 hours of water stress,respectively.
利用抑制差减杂交技术,分别构建小麦幼苗在水分胁迫1 h、6 h、12 h2、4 h和48 h条件下的cDNA文库,得到6 733条EST序列。
4)  suppression subtractive hybridization(SSH)
抑制差减杂交(SSH)
5)  Suppression subtractive hybridization (SSH)
抑制性差减杂交
1.
Suppression subtractive hybridization (SSH) was used to identification of differentially expressed genes in chicken kidney during avian influenza virus infection.
利用抑制性差减杂交技术(suppression subtractive hybridization,SSH)成功构建了禽流感病毒感染和正常对照鸡肾组织差异表达的双向差减cDNA文库,通过斑点杂交筛选到包括干扰素刺激基因(ISG12)、淋巴细胞抗原复合体基因(LY6E)、基质Gla蛋白基因 (MGP)、溶菌酶基因、IFN诱导的跨膜蛋白基因及一个没有任何同源信息的EST等6个表达上调基因;KIAA1259、谷光苷肽转移酶基因(GST)、酒精脱氢酶基因(ADH1)、突触结合蛋白基因(SytVx)、葡萄糖6磷酸酶催化哑基基因(G6PC)、钠/二羧酸转运蛋白基因(SDCT)、 MGC4504基因、MGC69686基因和一个没有任何同源信息的EST等9个表达下调基因。
6)  suppression subtractive hybridization
抑制差减杂交
1.
In order to study the genetic differences between epidemic and nonepidemic strains of Vibrio choleraeO1 E1 Tor, the suppression subtractive hybridization was used to screen for the different genes between epidemic and nonepidemic strains of V.
为了克隆与分析霍乱弧菌O1E1Tor流行株与非流行株两类菌株基因组差异片段 ,采用抑制差减杂交技术 (suppressionsubtractivehybridization ,SSH)分别以国内保留的流行株Wujiang 2及非流行株Js 32一株作为被检菌 ,另一株作为参考菌进行基因组差异研究 。
2.
To research salt tolerance mechanism and get the salt tolerance relative genes of Eustoma grandiflorum, a cDNA library was made by suppression subtractive hybridization technique.
为了研究草原龙胆抗盐性机理,初步获得其抗盐性相关基因,分别以400mmol·L-1的NaCl及清水处理的草原龙胆叶片mRNA为tester和driver方,利用抑制差减杂交技术构建了cDNA文库。
3.
Suppression subtractive hybridization (SSH) was carried out by using resistant pool as tester and susceptible pool as driver and SSH DNA library was constructed.
以YW642/中8601的F2代纯合的抗病和感病单株各10株提取DNA构建成近等基因池,以抗病池为试验方,以感病池为驱动方,进行抑制差减杂交(SSH),构建差减文库。
补充资料:减缘减行
【减缘减行】
 (术语)缘者,指上界四谛与上二界四谛之八谛。行者,指欲界四谛下十六行相与上二界四谛下十六行相之三十二行相。自声闻乘四善根之初至忍法之上忍,连环普观此上下八谛之三十二行相,同自中忍之位,一行减之,遂至留苦或道等之一行相,谓之减行,每减其四行相自减一谛,故谓之减缘。其所以减之者,泛观上下八谛之三十二行相,观智浮漫而不猛利,故自中忍以后渐渐狭其观境,以养成猛利之观智,遂为发真无漏智之算引也。上下八谛之三十二行相,第一回自第一观至第三十一,因而减去第三十二即上界道谛下出之一行相,第二回更自第一观至第三十,因而减去第三十一即上界道谛下行之一行相。如此每一周自下逆次减一行相,终至减第一即欲界苦谛下苦之一行相。即减去者,三十一行相也。减此三十一行相中,每第四周减一谛,故谓之减缘,减于他三周之行相,谓之减行。即减缘七周,减行二十四周,合于三十一周减去三十一行也。第四周谓之减缘者,上下八谛,为所缘之境,三十二行相,为对于此之能缘观解,故减四行相即减所缘之境一谛也。因而逆次减去,每至第四行相,不谓为减行,特附以所谓减缘即减谛之名耳。(参见:行相)
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条