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1)  gene-targeting vector construction
基因打靶载体构建
2)  gene targeting vectors
基因打靶载体
1.
To generate a loxPneoloxP selection cassette for construction of goat mammary gland gene targeting vectors, loxP sites were introduced into both the forward and reverse primers and a plasmid pTarget containing neo gene was used as the template for high fidelity PCR amplification.
为构建山羊乳腺基因打靶载体的选择标记盒,将loxP位点分别引入正、反向引物,以含新霉素磷酸转移酶(neo)基因的质粒pTarget为模板,用高保真聚合酶链反应(PCR)扩增得loxP neo loxP选择标记盒。
3)  human ribosomal DNA-targeting vector
核糖体基因区打靶载体
1.
For the reason of safety, our lab has developed a novel nonviral vector-human ribosomal DNA-targeting vector: pHrneo, which is a human derived vector and has been showed a capacity of targeting hFVIII gene into HT1080 cell and site-specific integration.
出于安全性方面的考虑,我们实验室已经构建了核糖体基因区打靶载体(human ribosomal DNA-target ing vector)—pHrneo,它是一种人源非病毒载体,可以靶向性地整合到人核糖体基因区,并且已经成功地利用该载体将外源基因人凝血因子Ⅷ导入人纤维肉瘤细胞(HT1080)中,获得定点整合细胞克隆。
4)  universal gene targeting vector
通用型基因打靶载体
5)  expression vector of CYP51 gene
CYP51基因载体构建
6)  gene targeting in somatic cell
体细胞基因打靶
补充资料:基因打靶法
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性质:又称同源重组技术(homelogens DNA technology)。是转基因方法的一种。利用一适当的DNA可在靶位相结合,使人们可在染色体的某个特定部位剔除或插进一个基因。可用来修饰一个现存基因,在器官移植中有用,还可用来发展某些人体遗传病的动物模型。

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