1) Targeting vector
打靶载体
1.
The results showed that Red/ET recombination has the advantage of high efficiency and time saving for construction of the targeting vector in studies of the gene function in streptomyces.
在Red重组酶的作用下,外源DNA片段与重组质粒pKC1139-AOP上的AHBA基因簇的同源区域重组,构建了AHBA基因簇打靶载体。
2.
Objective:To construct a replacement targeting vector for exon 2 of human CC chemokine ligand 20(hCCL20).
目的:构建针对人CC亚族趋化因子配体20(CC chemokine ligand20,CCL20)基因外显子2的置换型打靶载体。
2) gene targeting vectors
基因打靶载体
1.
To generate a loxPneoloxP selection cassette for construction of goat mammary gland gene targeting vectors, loxP sites were introduced into both the forward and reverse primers and a plasmid pTarget containing neo gene was used as the template for high fidelity PCR amplification.
为构建山羊乳腺基因打靶载体的选择标记盒,将loxP位点分别引入正、反向引物,以含新霉素磷酸转移酶(neo)基因的质粒pTarget为模板,用高保真聚合酶链反应(PCR)扩增得loxP neo loxP选择标记盒。
3) human ribosomal DNA-targeting vector
核糖体基因区打靶载体
1.
For the reason of safety, our lab has developed a novel nonviral vector-human ribosomal DNA-targeting vector: pHrneo, which is a human derived vector and has been showed a capacity of targeting hFVIII gene into HT1080 cell and site-specific integration.
出于安全性方面的考虑，我们实验室已经构建了核糖体基因区打靶载体(human ribosomal DNA-target ing vector)—pHrneo，它是一种人源非病毒载体，可以靶向性地整合到人核糖体基因区，并且已经成功地利用该载体将外源基因人凝血因子Ⅷ导入人纤维肉瘤细胞(HT1080)中，获得定点整合细胞克隆。
6) promoter-less knock-out vector
无启动子打靶载体
1.
Strategy of construction the prion promoter-less knock-out vector;
朊蛋白基因敲除无启动子打靶载体的构建策略
补充资料：打靶
1.按一定规则对设置的目标进行射击，以检验射击训练成效，提高射击技术。 2.方言。谓枪毙。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条