1) touchdown anchored PCR
温度降落锚式PCR
2) temperature fall,temperature drop
温度降落
3) Touch down PCR
降落PCR
1.
Methods:According to the Hsp70 cDNA sequence of Penaeus monodon accessed by GenBank,designed a pair of primers and used the optimal Touch Down PCR program to amplify the complete Hsp70 gene from genomic DNA of Litopenaeus vannamei.
方法:根据GenBank中斑节对虾(Penaeusmonodon)Hsp70基因的cDNA序列,设计引物,对经高盐法提取的凡纳滨对虾(Litopenaeusvannamei)基因组DNA,采用优化的降落PCR(TouchDownPCR)程序,扩增凡纳滨对虾Hsp70基因的全长序列。
4) TD PCR
降落PCR
1.
TD PCR——AN AMELIORATIVE PCR FOR AMPLIFICATION OF TARGET GENES;
一种优化的PCR方法——降落PCR扩增目的基因
2.
Target genes were amplified with the TD PCR method,two orthogonal experiments were designed to screen out optimal concentrations of various components of the PRC reaction system,while to explore annealing time,extending time and cycling frequency.
采用降落PCR方法扩增目的基因,设计两次正交试验对PCR反应体系中的各组分浓度进行筛选,同时对退火时间、延伸时间及循环次数进行摸索。
3.
Using the primers, the PVⅢ gene was amplified by touchdown PCR (TD PCR).
1kb左右的引物 ,采取降落PCR对EDSV gDNA进行扩增。
5) touchdown PCR
降落PCR
1.
Modified touchdown PCR as an approach to amplify target gene of Cricetulus griseus;
改良的降落PCR扩增中国地鼠目的基因
2.
The regenerated sugar beet plant of the third generation is detected by touchdown PCR.
采用降落PCR的方法对第三代转基因甜菜进行了检测。
3.
Many strategies were used in this method including TdT tailing, anchored PCR, nested PCR, touchdown PCR.
以龙眼为材料,采用TdT(末端脱氧核苷酸转移酶)加尾、锚定PCR、巢式PCR、降落PCR等多种策略。
6) TD-PCR
降落PCR
1.
Touchdown PCR (TD-PCR) was done and among the products, 19 fragments with 800~1 000 bp in length were selected for sequence analysis.
富集培养胞外蛋白酶产生菌并提取了12个总DNA样品,分别用每对引物在降落PCR (TouchdownPCR, TD-PCR)条件下进行蛋白酶编码序列的扩增。
2.
Touchdown polymerase chain reaction (TD-PCR) was applied to detect the genetically modified components in soybeans for the first time.
采用改良的CTAB法,植物基因组DNA小量制备试剂盒,SDS法和高盐低pH值法4种方法提取安徽市场上8种大豆种子的基因组DNA,首次用降落PCR(TD-PCR)检测转基因成分,并对阳性产物进行酶切验证。
补充资料：多重锚式基团
分子式：
CAS号：
性质：在超分散剂分子结构中，为了更牢固吸附或键合在颜料粒子表面上，减少分散剂从粒子表面上解吸，设计的分子通常具有多个锚式基团如-SOO3H，-COOH等，即多重锚式基团，此时尽管单一锚式基团结合力较低，但通过多点弱结合力的综合作用，仍可较牢固地结合在粒子表面。
CAS号：
性质：在超分散剂分子结构中，为了更牢固吸附或键合在颜料粒子表面上，减少分散剂从粒子表面上解吸，设计的分子通常具有多个锚式基团如-SOO3H，-COOH等，即多重锚式基团，此时尽管单一锚式基团结合力较低，但通过多点弱结合力的综合作用，仍可较牢固地结合在粒子表面。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条