1) Arbitrary degenerate (AD) primers
随机简并(AD)引物
2) degenerate primer
简并引物
1.
Application of degenerate primers in cloning hsp70 gene families of Dunaliella salina;
简并引物在克隆盐藻热休克蛋白70基因家族中的应用
2.
Methods:A pair of degenerate primers were designed according to the homologous amino acid conserved sequences of EWYGWHF and KYGLIYQ from known MAR-binding protein and used to amplify MAR-binding protein cDNA fragment from Dunaliella salina by PCR technique.
方法:根据多种生物MAR结合蛋白氨基酸的高度保守序列EWYGWHF和KYGLIYQ,设计一对简并引物,以杜氏盐藻的cDNA为模板,进行PCR扩增,PCR产物与T载体相连,转化大肠杆菌JM109后,筛选鉴定,对阳性菌落进行测序分析。
3.
Methods:A pair of degenerate primers was designed according to the conserved motifs of SVAYKNV and DSTLIMQ of homologous amino acid sequences, and total RNA of Dunaliella salina was extracted using TRIzol reagent.
方法:根据衣藻、烟草、豌豆、拟南芥、西红柿等14-3-3蛋白的高度保守序列SVAYKNV、DSTLIMQ设计一对简并引物,采用RT PCR方法扩增杜氏盐藻14-3-3蛋白的cDNA片段。
3) Degenerate primers
简并引物
1.
Detection of Lily mottle virus by RT-PCR using special primers and degenerate primers;
应用特异引物和简并引物检测百合斑驳病毒
2.
Methods Degenerate primers were designed and then gradient PCR was used to decide the optimization annealing temperature.
目的 建立一种筛选第一、二和三类整合酶基因 (intⅠ )的简并引物PCR方法 ,并对临床菌株进行整合子筛选和分类。
3.
Objective To amplify and clone expressed sequence tags(ESTs) of genes encoding proteins with TF-ⅢA motif using degenerate primers.
目的 应用简并引物RT-PCR扩增并克隆TF-ⅢA型锌指蛋白基因表达序列标签（EST），以最终获得新的锌指蛋白基因。
4) accidental degeneracy
随机简并度
5) degenerate oligonucleotide-primer
简并性引物
补充资料：寡核苷酸随机引物
分子式：
CAS号：
性质：为一组6个核苷酸随机组成的寡聚物，几乎包含所有6核苷酸的序列。在基因工程技术中常用来作目的片段探针的制备。
CAS号：
性质：为一组6个核苷酸随机组成的寡聚物，几乎包含所有6核苷酸的序列。在基因工程技术中常用来作目的片段探针的制备。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条