1) Special degenerate primers
特异性简并引物
2) degenerate oligonucleotide-primer
简并性引物
3) degenerate primer
简并引物
1.
Application of degenerate primers in cloning hsp70 gene families of Dunaliella salina;
简并引物在克隆盐藻热休克蛋白70基因家族中的应用
2.
Methods:A pair of degenerate primers were designed according to the homologous amino acid conserved sequences of EWYGWHF and KYGLIYQ from known MAR-binding protein and used to amplify MAR-binding protein cDNA fragment from Dunaliella salina by PCR technique.
方法:根据多种生物MAR结合蛋白氨基酸的高度保守序列EWYGWHF和KYGLIYQ,设计一对简并引物,以杜氏盐藻的cDNA为模板,进行PCR扩增,PCR产物与T载体相连,转化大肠杆菌JM109后,筛选鉴定,对阳性菌落进行测序分析。
3.
Methods:A pair of degenerate primers was designed according to the conserved motifs of SVAYKNV and DSTLIMQ of homologous amino acid sequences, and total RNA of Dunaliella salina was extracted using TRIzol reagent.
方法:根据衣藻、烟草、豌豆、拟南芥、西红柿等14-3-3蛋白的高度保守序列SVAYKNV、DSTLIMQ设计一对简并引物,采用RT PCR方法扩增杜氏盐藻14-3-3蛋白的cDNA片段。
4) Degenerate primers
简并引物
1.
Detection of Lily mottle virus by RT-PCR using special primers and degenerate primers;
应用特异引物和简并引物检测百合斑驳病毒
2.
Methods Degenerate primers were designed and then gradient PCR was used to decide the optimization annealing temperature.
目的 建立一种筛选第一、二和三类整合酶基因 (intⅠ )的简并引物PCR方法 ,并对临床菌株进行整合子筛选和分类。
3.
Objective To amplify and clone expressed sequence tags(ESTs) of genes encoding proteins with TF-ⅢA motif using degenerate primers.
目的 应用简并引物RT-PCR扩增并克隆TF-ⅢA型锌指蛋白基因表达序列标签（EST），以最终获得新的锌指蛋白基因。
6) Specific primer
特异性引物
1.
According to the result of clone and sequence analysis,a specific primer was designed and used to identify tissue culture seedlings of D.
为了能方便快捷地鉴别出铁皮石斛试管苗和铁皮石斛,采用随机扩增多态性DNA(RAPD)技术对铁皮石斛试管苗及石斛属23个种进行了基因组DNA多态性分析,从中找出铁皮石斛试管苗特征性条带进行克隆和测序,并根据测序结果设计了特异性引物。
2.
Objective: To analyze the genetic relationship of Dendrobium and design a specific primer to differentiate Dendrobium candidum effectively.
目的 :对石斛属植物亲缘关系进行分析 ,并设计一对能方便准确地鉴别铁皮石斛的特异性引物。
补充资料：周期性特异性药物
周期性特异性药物
仅能杀灭*细胞增殖周期中某一期癌细胞的抗癌药物。依其作用又分为两类：①作用于S期药物，如*羟基脲、*阿糖胞苷，甲氨蝶呤、巯基嘌呤、硫鸟嘌呤等。其中前两种药物特异性较高，主要干扰DNA合成，但因也能干扰 RNA的合成，可能起到一定的自限作用。临床上宜缓慢静滴或肌注给药。②作用于M期的药物，如长春新碱等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条