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1)  a-little-amount-extraction-in-base method
碱法少量提取法
1.
In this experimentol study,a-little-amount-extraction-in-base method,kit method,and simple and quick boil method are used to extract plasmid pCB302-3.
实验采用简化的碱法少量提取法、简便快速的煮沸法及试剂盒法提取质粒 p CB30 2 -3。
2)  alkali extraction
碱法提取
1.
The optimum conditions for alkali extraction were as follows: pH 10,temperature 40℃,ratio of water to stuff 10 ∶1,extraction time 60 min,and the extraction rate of protein was 75.
碱法提取棉籽蛋白的最佳工艺为:pH 10,温度40℃,液料比10∶1,提取时间60 m in,此时蛋白提取率为75。
3)  separated method
碱提取法
4)  alkali-enzymatic method
碱-酶法提取
1.
To optimize the extraction technology of β-(1,3)-D-glucan from beer waste yeast with alkali-enzymatic method,orthogonal test was applied based on the single-factor experiments with dry yeast powder as raw material and extract rate of β-(1,3)-D-glucan,protein content and polysaccharid content were taken into account in this paper.
以啤酒酵母粉为原料,综合考虑提取率、蛋白质含量和多糖含量3个指标,在单因素实验的基础上,采用正交实验,得出碱-酶法提取废啤酒酵母中β-(1,3)-D-葡聚糖的理想工艺条件:酵母粉经过水洗,脱色后,按照150mL∶10g的比例加入3%的NaOH溶液,在80℃水浴条件下水解2h,离心,洗涤,调整pH至8。
5)  ethanol-alkali extraction method
醇碱提取法
1.
This paper deals with the ethanol-alkali extraction method of astragalus polysaccharides,by which the effects of five extraction factors including pH of extraction solvent,the ratio of material to extraction(solvent,)(extraction time,) extraction temperature and ethanol concentration of extraction solvent on the rate of extraction were studied.
采用醇碱提取法提取黄芪多糖,通过单因素实验设计分别研究了提取溶剂pH值、料液比、提取时间、提取温度和提取溶剂乙醇浓度5种因素对黄芪多糖提取率的影响,利用紫外(可见)分光光度法和红外光谱法对醇碱提取法提取黄芪多糖的含量进行检测,计算黄芪多糖的提取率为19。
6)  alkaline solution extraction technology
碱液提取法
补充资料:碱法明胶
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性质:又称灰法明胶,B型明胶。用碱法工艺制得的明胶。皮料或骨料先经一系列预处理,除去无用物质,提出能够生产明胶的胶原——皮胶原或骨胶原(骨素)。碱法制胶是用石灰乳处理胶原,亦即浸灰,使其转化为明胶。由于浸灰条件缓和,灰液浓度低(2%~5%氢氧化钙悬浮液),温度控制在20℃以下,浸灰时间长达20周左右,在转化过程中水解作用缓慢,不会过多地损害肽链,除去杂质也较彻底,因此可以获得高产率、高质量的明胶。碱法明胶的特点是含氮量和等电点都偏低(相应地为<18%,pH值为4.7~5.2)。活性杂质含量少,理化性能好。感光工业用的照相明胶大都为碱法明胶,特别是碱法骨明胶。

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