1) expression in silkworm cells (Bombyx mori
家蚕细胞表达
2) silkworm (expression
家蚕表达
3) BmN cells
家蚕细胞
1.
The hLF gene was inserted into the baculovirus transfer vector pBacPAK_8, and the recombinant plasmid pBacPAK-hLF was co-transfected with linearized Bm-BacPAK_6 virus DNA into BmN cells, then homologous recombination occurred inside the cells.
该质粒DNA与已线性化BacPAK6DNA共转染家蚕细胞BmN,在培养的贴壁细胞中挑出空斑,经过3轮纯化,获得重组病毒BmNPV-hLF。
2.
In order to deal with this problem,we cloned the poly gene with its promoter of Bombyx mori nucleopolyhedrovirus(BmNPV) into pigA3GFP vector,and transported it into BmN cells by lipofectamine with helper vector pHA3PIG.
将家蚕核型多角体病毒(Bombyx mor inucleopolyhedrovirus,BmNPV)中的多角体基因(polyhedrin,poly)及其启动子片段克隆到转座子载体pigA3GFP中,将其与辅助质粒pHA3PIG利用脂质体介导法导入家蚕细胞中,经过多次筛选获得稳定的转基因家蚕细胞。
3.
Constructed vector was coinfected with linear Bm BacPAK6 DNA into BmN cells.
与线形化Bm BacPAK6病毒基因组DNA共转染家蚕细胞,经过体内重组,筛选到重组病毒Bm BacPAK IFN THY。
4) BmN cell
家蚕细胞
1.
The recombinant BmNPV(rBmHBe) was used to study the optimal conditions for expression of HBeAg gene in BmN cell.
利用已构建的含有HBeAg基因的重组家蚕核多角体病毒rBmHBe ,摸索了HBeAg在家蚕细胞中表达的最佳条件组合 ,细胞接种密度 :(3 。
6) Silkworm/BmNPV expression system
家蚕/BmNPV表达系统
补充资料：家蚕
| 家蚕 Bombyx mori 见桑蚕。 |
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条