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1)  binary vector con-struction
双元载体构建
2)  Construction of binary vectors
双元表达载体构建
3)  binary vector
双元载体
1.
Introduction of kanamycin resistant gene into tomato somatic cells by Ti plasmid binary vector system;
用Ti质粒的双元载体法将抗性基因转入西红柿体细胞
2.
The appropriate products of the Pi36-1 and Pi36-2 were purified and then ligated into the binary vector of pCAMBIA1300,as well as that of the Pi36-3 ligated into the binary vector of pLYTAC27 and the reconstructed pCAMBIA1300AscI,respectively.
5kb,电泳回收目的片段,将Pi36-1和Pi36-2分别克隆到双元载体pCAMBIA1300,而将Pi36-3克隆到双元载体pYLTAC27的AscI位点。
3.
The 56KD protein gene & 3 part non encoded region of the PLRV Chinese isolates which contained in plasmid pLR56 were constructed into binary vector plasmid pROK Ⅱ by Two-step link method, which was introduced into the agrobacterium tumefaciens LBA4404 by tri-parental conjugation and direct entering ways.
为培育转基因抗马铃薯卷叶病毒的新材料 ,采用二次连接法将存在于克隆载体 p L R5 6中的马铃薯卷叶病毒 5 6 KD蛋白基因及 3 端非编码区构建到双元载体系统的中间表达载体质粒p ROK 中 ,采用三亲融合法和直接导入法转化根癌农杆菌 LBA4 40 4 ,PCR法检测。
4)  Vector construction
载体构建
1.
Cloning and eukaryotic expression vector construction of mouse FasL gene and its expression in dendritic cells;
小鼠FasL全长cDNA的克隆、真核表达载体构建及其在树突状细胞中的表达
2.
Codon optimization of SMAP-29 gene and its expression vector construction in Pichia pastoris;
抗菌肽SMAP-29基因密码子优化及其毕赤酵母表达载体构建
5)  construction of vector
载体构建
6)  Vector construct
载体构建
补充资料:2,2-双氯甲基-三亚甲基-双[双(2-氯乙基)磷酸脂]
CAS:38051-10-4
分子式:C13H24Cl6O8P2
中文名称:2,2-双氯甲基-三亚甲基-双[双(2-氯乙基)磷酸脂]

英文名称:2,2- bis(chloromethyl)-trimethylene bis[bis(2-chloroethyl)phosphate]
phosphoric acid, 2,2-bis(chloromethyl)-1,3-propanediyl tetrakis(2-chloroethyl)
2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate)
2,2-bis(chloromethyl)-1,3-propanediyltetrakis(2-chloroethyl)phosphate
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