2) FAD2-RNAi vector
FAD2-RNAi载体
3) RNAi vector
RNAi载体
1.
The produced fragments were respectively introduced to the binary expressed vector pBI121 to produce pBI-PhrIP1 and to the GFP expressed vector pMON18342 to produce pMON-GFP-PhrIP1 and to the RNAi vector Hellsgate2 to produce Hellsgate2-PhrIP1,and then these plasmids were respectively mo.
216kb的片段,分别克隆至双元表达载体、GFP载体和RNAi载体上,得到了植物超表达载体pBI-PhrIP1、GFP载体pMON-GFP-PhrIP1和RNAi载体Hellsgate2-PhrIP1。
2.
The produced fragments were respectively introduced to the binary expressed vector pBI121 to produce pBI-Ran2 and RNAi vector Hellsgate2 to produce Hellsgate2-Ran2.
根据编码拟南芥小GTP结合蛋白的Ran2基因cDNA全序列设计超表达引物和序列内部第397~610bp之间序列设计RNAi引物,以pMD18-T-Ran2为模板,用PCR方法分别扩增出666bp和214bp的片段,分别连接至双元表达载体和RNAi载体上,得到了植物超表达载体pBI-Ran2和RNAi载体Hellsgate2-Ran2,并用电转化法导入农杆菌GV3101菌株中,PCR扩增结果表明所构建的植物超表达载体pBI-Ran2和RNAi载体Hellsgate2-Ran2已导入农杆菌。
3.
In order to efficiently identify the functions of ZmPti1 gene,two plant-transformation vectors,pBPC-ZmPti1-bar(sense expression vector)and pBPC-PtS-GFP-PtAs-bar(RNAi vector)were prepared.
构建了ZmPti1基因的正义表达载体和RNAi载体,以bar基因为抗性筛选标记,通过花粉管通道法将构建的两个表达载体分别转化到玉米自交系178。
6) Vector construction
载体构建
1.
Cloning and eukaryotic expression vector construction of mouse FasL gene and its expression in dendritic cells;
小鼠FasL全长cDNA的克隆、真核表达载体构建及其在树突状细胞中的表达
2.
Codon optimization of SMAP-29 gene and its expression vector construction in Pichia pastoris;
抗菌肽SMAP-29基因密码子优化及其毕赤酵母表达载体构建
补充资料：[3-(aminosulfonyl)-4-chloro-N-(2.3-dihydro-2-methyl-1H-indol-1-yl)benzamide]
分子式：C16H16ClN3O3S
分子量：365.5
CAS号：26807-65-8
性质：暂无
制备方法：暂无
用途：用于轻、中度原发性高血压。
分子量：365.5
CAS号：26807-65-8
性质：暂无
制备方法：暂无
用途：用于轻、中度原发性高血压。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条