1)  cloning
基因克
2)  gene clone
基因克隆
1.
Study and development on isolation,purification, gene clone and functions of lectins;
凝集素的分离纯化、基因克隆及功能研究进展
2.
Gene Clone and HighLevel Expression of Staphylokinase;
葡萄球菌激酶(Sak)的基因克隆和高效表达工程菌的构建
3.
To explore the effect of a tyrosine kinase inhibitor STI571 and P21 WAF gene clone on the proliferation, cycle, apoptosis of leukemia cell line K562, P21 WAF gene was obtained by RT PCR, and its sequence was approved to be correct, then P21 pcDNA3.
本研究探讨酪氨酸激酶抑制剂STI5 71和P2 1WAF基因克隆对慢性粒细胞白血病急变K5 62细胞株的细胞增殖、细胞周期及凋亡等的治疗作用。
3)  gene cloning
基因克隆
1.
Study on gene cloning of Chlamydial pneumonia CPn0308 and its endogenous localization;
肺炎衣原体CPn0308的基因克隆及其内源性蛋白定位的研究
2.
Gene cloning of glycoprotein of Hantavirus and construction of G1 and G2 glycoprotein baculovirus expression vectors;
汉坦病毒G1和G2糖蛋白的基因克隆及糖蛋白杆状病毒表达载体的构建
3.
Gene cloning, expression and purification of Shiga like toxin 2B from EHEC O157∶H7;
EHECO157∶H7志贺样毒素Ⅱ结合亚单位Stx2B的基因克隆、表达与纯化
4)  clone
基因克隆
1.
Tuberculosis was amplified by PCR and the target gene we got was cloned into the unique HindⅢ and EcoRⅠcloning sites of pcDNA3.
4(MS)进行基因克隆,构建其真核表达质粒并加以鉴定。
2.
In this study,the coat protein gene of GLRaV 3 was cloned into expression vector PET 30a,then translated into E.
将葡萄卷叶伴随病毒 的 CP基因克隆到表达载体 p ET- 30 a中 ,转化大肠杆菌 BL2 1,筛选得到阳性克隆 GB- 1,用 IPTG诱导使其表达。
5)  molecular cloning
基因克隆
1.
In this article,the biological function,molecular cloning,genetic engineering and expression in body of lysozyme were summarized.
综述了水生动物包括鱼、虾和贝类溶菌酶的生物学功能,溶菌梅的基因克隆、基因工程表达及其在机体内的表达规律等的研究进展,并探讨了其在水产养殖病害防治方面的应用前景。
6)  cloning
基因克隆
1.
Molecular Cloning and Sequencing of Chinese Phospholipicl Transfer Protein Gene;
中国人磷脂转运蛋白基因克隆及序列分析
2.
Cloning and Characterization of β-Carotene Ketolase Gene from Haematococcus pluvialis and Construction of its Chloroplast Transformation Expression Vector;
雨生红球藻β-胡萝卜素酮化酶基因克隆、分析及叶绿体表达载体构建
3.
Cloning and Expression of D-Lactate Dehydrogenase Gene in Lactobacillus sp. MD-1;
D-乳酸脱氢酶基因克隆及其表达
参考词条
补充资料：基因克隆
分子式：
CAS号：

性质： 把基因与载体经体外连接成一新的能产生多个复制体的重组分子，转化新的宿主，并在其内能产生多个复制体，且能采用大量培养的技术。大肠杆菌常被用作达到这一目的宿主生物。基因克隆有时也用来代替基因工程或基因操作这一术语。克隆是来自一个共同的祖先，具有完全相同的子代群体。通过克隆技术产生大量相同的DNA分子或特定基因，目的在于进行鉴定或用于其他场合(如用于改造生物的某些遗传特性等)。这一技术的基本操作包括：(1)选择合适的载体(如细菌质粒、噬菌体、柯斯质粒等)构建重组DNA分子；(2)将重组体转移入宿主细胞(如大肠杆菌)，重组体进行复制；(3)筛选所需的基因克隆，可以获得大量纯一的基因拷贝。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。