1) gene cloning
基因克隆化
2) cloned gene
克隆化基因
3) gene clone
基因克隆
1.
Study and development on isolation,purification, gene clone and functions of lectins;
凝集素的分离纯化、基因克隆及功能研究进展
2.
Gene Clone and HighLevel Expression of Staphylokinase;
葡萄球菌激酶(Sak)的基因克隆和高效表达工程菌的构建
3.
To explore the effect of a tyrosine kinase inhibitor STI571 and P21 WAF gene clone on the proliferation, cycle, apoptosis of leukemia cell line K562, P21 WAF gene was obtained by RT PCR, and its sequence was approved to be correct, then P21 pcDNA3.
本研究探讨酪氨酸激酶抑制剂STI5 71和P2 1WAF基因克隆对慢性粒细胞白血病急变K5 62细胞株的细胞增殖、细胞周期及凋亡等的治疗作用。
4) gene cloning
基因克隆
1.
Study on gene cloning of Chlamydial pneumonia CPn0308 and its endogenous localization;
肺炎衣原体CPn0308的基因克隆及其内源性蛋白定位的研究
2.
Gene cloning of glycoprotein of Hantavirus and construction of G1 and G2 glycoprotein baculovirus expression vectors;
汉坦病毒G1和G2糖蛋白的基因克隆及糖蛋白杆状病毒表达载体的构建
3.
Gene cloning, expression and purification of Shiga like toxin 2B from EHEC O157∶H7;
EHECO157∶H7志贺样毒素Ⅱ结合亚单位Stx2B的基因克隆、表达与纯化
5) clone
[英][kləʊn] [美][klon]
基因克隆
1.
Tuberculosis was amplified by PCR and the target gene we got was cloned into the unique HindⅢ and EcoRⅠcloning sites of pcDNA3.
4(MS)进行基因克隆,构建其真核表达质粒并加以鉴定。
2.
In this study,the coat protein gene of GLRaV 3 was cloned into expression vector PET 30a,then translated into E.
将葡萄卷叶伴随病毒 的 CP基因克隆到表达载体 p ET- 30 a中 ,转化大肠杆菌 BL2 1,筛选得到阳性克隆 GB- 1,用 IPTG诱导使其表达。
3.
Objective:To clone,express and identify the gene of Gal d 3 in eggs.
设计带有酶切位点的特异性引物,采用RT- PCR获得整个短的开放阅读框并将目的基因克隆到大肠杆菌表达载体pET-28a中进行诱导表达。
6) molecular cloning
基因克隆
1.
In this article,the biological function,molecular cloning,genetic engineering and expression in body of lysozyme were summarized.
综述了水生动物包括鱼、虾和贝类溶菌酶的生物学功能,溶菌梅的基因克隆、基因工程表达及其在机体内的表达规律等的研究进展,并探讨了其在水产养殖病害防治方面的应用前景。
补充资料：克隆化
克隆化
cloning
将目的基因与运载体结合后，通过转化引入受体细胞(多用大肠杆菌或枯草杆菌或酵母菌）。使用选择性培养基来筛选出已转化细菌，将筛选出的菌液大量繁殖，这种获取单个细胞培养的过程，通称为克隆化。即细胞通过克隆化可获得“单克隆”细胞系。其目的是分出目的基因细胞，防止竞争淘汰和外来干扰。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条