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1)  Restriction fragment length polymor phisms(RFLP)
DNA限制性片段长度多态性(RPLP)
2)  restriction fragment length polymorphism of mitchondrial DNA
线粒体DNA限制性片段长度多态性分析
1.
fulviflamma) were studied comparatively, which were based on 2 methods: restriction fragment length polymorphism of mitchondrial DNA (mtDNA-RFLP), RFLP of cytochrome b (Cyt b) gene segment(>900bp) amplified by polymerase chain reaction(PCR-RFLP).
采用线粒体DNA限制性片段长度多态性分析(mtDNARFLP)和线粒体DNA的细胞色素b基因(Cytb)的部分序列扩增限制性片段长度多态性分析(mtDNAPCRRFLP)两种方法,对笛鲷属5种鱼类进行种间系统发育的比较研究,结果显示:(1)画眉笛鲷依照其形态学上体侧条带颜色差异,可分为褐带画眉笛鲷和黄带画眉笛鲷两个种;(2)千年笛鲷和星点笛鲷、褐带画眉笛鲷和黄带画眉笛鲷、金带笛鲷和金焰笛鲷之间遗传距离相对较近;(3)两种分析方法都可以为种的区分提供方便有效的分子标记;(4)两种分析方法在构建发育系统树上不完全一致。
3)  restriction fragment length polymorphism of mitochondrial DNA (mtDNA RFLP)
线粒体DNA限制性片段长度多态性
4)  restriction fragment length polymorphism
限制性片段长度多态性
1.
Identification of 60 strains of clinical isolated Microsporum canis with restriction fragment length polymorphism
60株临床分离犬小孢子菌的聚合酶链反应-限制性片段长度多态性鉴定
2.
The research advance on the detection methods of beer-spoilage microorganisms is reviewed,including polymerase chain reaction,restriction fragment length polymorphism,immunity technology,nucleic acid hybrid-ization and,voltammetric biosensor.
内容包括聚合酶链式反应,限制性片段长度多态性,免疫学技术,核酸杂交,伏安型生物传感器。
3.
Methods HBV genotypes were determined by the restriction fragment length polymorphism analysis in 120 HBV DNA PCR positive patients infected with hepatitis B,including 17 asymptomatic.
方法选择宁夏回族HBVDNA阳性慢性乙肝病人共120例,其中病毒携带者(ASC)17例,慢性肝炎(CH)34例,肝硬化(LC)58例,肝细胞癌(HCC)11例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测HBV基因型。
5)  RFLP
限制性片段长度多态性
1.
Objective To establish a PCR-RFLP method for detection of CD26 mutation of hemoglobin E(HbE)gene.
目的建立PCR-RFLP(限制性片段长度多态性)法检测血红蛋白E(HbE)基因CD26突变。
2.
The point mutation of pre-C 1896-position was detected by PCR and restriction fragment length polymorphism (PCR-RFLP).
方法用荧光定量PCR检测64例HBeAg阴性HBV感染者血清HBVDNA,ELISA法检测preS1抗原,以错配聚合酶链反应结合限制性片段长度多态性(PCR-RFLP)分析HBV前C区1896位点的基因变异。
3.
Methods Genomic DNA was analyzed for the mutation allele in 100 healthy controls by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对100名上海地区汉族健康者PAF-AH4号外显子275位点G/A位点的SNP进行了检测,计算其基因型和等位基因频率,并结合文献进行了不同种族间的分析比较。
6)  Restriction fragment length polymorphisms
限制性片段长度多态性
1.
Identification of 4 Trichinella isolates by Restriction Fragment Length Polymorphisms analysis;
猪、犬旋毛虫DNA限制性片段长度多态性分析
2.
Methods:Restriction fragment length polymorphisms(RFLP)at PstI、SstI sites of ApoAI-CIII-AIV gene cluster were studied using polymerase chain reaction(PCR)in 161 patients with cholesterol gallstone and 94 healthy subjects from a Chinese population in Sichuan Province.
方法应用多聚酶链反应技术(PCR)对161例胆固醇结石病患者及94例正常人载脂蛋白(Apo)AI-CIII-AIV基因簇的限制性片段长度多态性(RFLP)进行研究,检测ApoAI-CIII-AIV基因位点PstI、SstI。
3.
Methods Lipoprotein lipase gene Hind Ⅲ polymorphism was studied using polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) in 103 type Ⅱb hyperlipoproteinemia patients and 129 healthy subjects from a population of Chinese Hans in Chengdu area.
方法 采用聚合酶链反应及限制性片段长度多态性方法对成都地区 10 3例 b型高脂蛋白血症患者及 12 9名血脂正常者脂蛋白脂酶基因 Hind 酶切位点的多态性及其与血脂、载脂蛋白水平的关联进行了研究。
补充资料:限制性片段长度多态性
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性质:又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点,经酶作用后,在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化,则酶切位点或数目可能有差异,则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。

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