1) plastid transformation vector
叶绿体转化载体
2) chloroplast transformation
叶绿体转化
1.
Some molecular strategies for controlling transgene escape were summarized, which included chloroplast transformation, male sterility, terminator technology, incompatible genomes, GM "gene deletor" The advantage and problem of these strategies were analysed, and then some constructive countermeasures and proposal to preventing transgene escape in the future were put forward.
综述了几种防范转基因逃逸的分子策略:叶绿体转化法、雄性不育法、终止子技术、染色体组特异性选择、外源基因删除法,分析了这几种方法的优势及存在的问题,并对将来如何更好地防范植物转基因逃逸提出了一些建设性的对策及建议。
2.
In order to eliminate the maintain-line seeds from the cytoplasm male sterile line seeds a selectable marker gene was inserted into the cytoplasmic genome of the male sterile line through chloroplast transformation.
根据已发表的大豆叶绿体序列(GenBankX07675)设计引物,用PCR方法获得两段大豆叶绿体同源重组序列LHRR和RHRR,克隆到质粒pUC19中,并将来源于质粒pTF102的壮观霉素抗性基因aadA克隆到两段同源重组序列之间,以构建大豆叶绿体转化表达载体pJY01。
3.
The text has surveyed Crop genetic engineering from several respects such as resistance to herbicide, disease beet resistance,drought tolerance, and that it has been reviewed the progress of directly making use of genetic engineering to improve the quality and yield of the crop, and the new advance in chloroplast transformation was introduced.
综述了国内外抗除草剂、抗病、抗虫、抗逆境的作物基因工程,以及直接利用基因工程的手段改良农作物的品质、产量的研究进展,并介绍了叶绿体转化基因工程的最新进展。
3) Site directed chloroplast transformation
叶绿体定点转化
补充资料:叶绿体核糖体
分子式:
CAS号:
性质:真核生物叶绿体中发现的70s核糖体。在70s核糖体上合成蛋白质可被氯霉素抑制,而不受环己酰亚胺(放线酮)影响,这情况正好与(真核生物)80s细胞质核糖体相反。叶绿体的核糖体可分为50s及30s的亚基。在70s核糖体中链的引发需要用N-甲酰甲硫氨酸而不是甲硫氨酸。大约有50种蛋白质与70s核糖体有关,其中有几种系由细胞质系统合成的。
CAS号:
性质:真核生物叶绿体中发现的70s核糖体。在70s核糖体上合成蛋白质可被氯霉素抑制,而不受环己酰亚胺(放线酮)影响,这情况正好与(真核生物)80s细胞质核糖体相反。叶绿体的核糖体可分为50s及30s的亚基。在70s核糖体中链的引发需要用N-甲酰甲硫氨酸而不是甲硫氨酸。大约有50种蛋白质与70s核糖体有关,其中有几种系由细胞质系统合成的。
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参考词条