Some molecular strategies for controlling transgene escape were summarized, which included chloroplast transformation, male sterility, terminator technology, incompatible genomes, GM "gene deletor" The advantage and problem of these strategies were analysed, and then some constructive countermeasures and proposal to preventing transgene escape in the future were put forward.

In order to eliminate the maintain-line seeds from the cytoplasm male sterile line seeds a selectable marker gene was inserted into the cytoplasmic genome of the male sterile line through chloroplast transformation.

The text has surveyed Crop genetic engineering from several respects such as resistance to herbicide, disease beet resistance,drought tolerance, and that it has been reviewed the progress of directly making use of genetic engineering to improve the quality and yield of the crop, and the new advance in chloroplast transformation was introduced.

From methyl pheophorbide-a (MPa, 1), the pheophorbide-a derivative substituted with hydro- pyrazol 2 was obtained by the 1,3-dipolar cycloaddition of vinyl group at 3-position with diazomethane.