1) isogenic DNA
基因纯化DNA
2) Isolation and purity genomic DNA
基因组DNA提纯
3) global DNA methylation
基因组DNA甲基化
1.
A sensitive and precise method for the quantification of global DNA methylation level in human colon cancer tissues was developed by liquid chromatography-electrospray ionization tandem mass spectrometry with multiple reaction monitoring(MRM) mode under optimized conditions.
利用多重监测反应模式(MRM),通过对液相色谱及质谱条件的优化,建立了人体结肠癌组织样品中基因组DNA甲基化的高效液相色谱-电喷雾质谱(HPLC-ESIMS/MS)检测方法,对结肠癌组织及癌旁组织中基因组DNA甲基化进行了检测。
4) DNA Purification
DNA纯化
1.
Sequence based typing (SBT) becomes the best method of HLA typing because of development of DNA amplification, DNA purification and other technology from 1 990 s, SBT is gold standard of HLA gene typing on organ transplantation, human genetics and disease association.
20世纪90年代以来DNA扩增、DNA纯化和人类基因成果对HLA领域的渗透,使得碱基序列测序方法(sequencebasedtyping,SBT)成为一种可直接阅读HLA系统复杂性的基因物质的最佳技术,并在器官移植、人类遗传学、疾病关联等研究方面被认为是HLA基因分析的金标准。
2.
DNA extraction includes two steps of cell lysis and DNA purification.
DNA提取分细胞裂解和DNA纯化2步,对细胞裂解比较了珠磨匀浆法、反复冻融法、十二烷基磺酸钠(sodium dodecyl sul-fate,SDS)裂解法等7种方法;对DNA纯化比较了酚/氯仿纯化法和胶回收纯化法。
5) Crude DNA purification
粗DNA纯化
补充资料:DNA分子花窗(沿B―DNA轴线方向看)
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说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条