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1)  AGP
琼扩试验
1.
The ReoS1133 was inoculated into chorioallantoic chamber or allantoic cavity of 10-day-old embryonated chick eggs,allantoic fluid of embryos died in 48 h-120 h was collected,deactivated and concentrated to get AGP antigen;Killed avian reovirus vaccine made by the antigen was immunized into SPF fowl twice to get positive serum;Negative serum can be got from SPF fowl.
经过效价测定制备16单位琼扩抗原,8单位阳性血清;取免后的SPF鸡血样4个20~2-5稀释分别与1单位~8单位琼扩抗原做琼扩试验,确定了应该用4单位或8单位琼扩抗原做工作抗原;并以血清中和试验为参照证明琼扩试验在血清抗体监测中的可行性。
2)  AGP
琼脂扩散试验
1.
The paper introduces the establishment methods of three determination techniques(AGP,FA and PCR) for detecting goose parvovirus infection.
利用鹅细小病毒贵州分离株制备琼脂扩散试验沉淀抗原,建立琼脂扩散试验方法;以该分离株免疫家兔、制备高免血清、提取IgG制备荧光抗体,建立荧光抗体检测技术;根据鹅细小病毒VP3基因设计一对引物,建立PCR检测技术;然后采用这3种技术对鹅细小病毒感染临床病例进行检测比较,结果表明,这3种诊断技术均具有较高的特异性,可用于鹅细小病毒感染的临床诊断,但以荧光抗体检测技术的敏感性最高。
2.
The results of ELISA and agar gel precipition(AGP)were compared.
通过对不同来源的鸡传染性喉气管炎病毒(ILTV)血清的检测,进行ELISA试剂盒检测ILTV抗体的应用研究,并与传统的琼脂扩散试验(AGP)方法进行了比较。
3.
40 blind samples were examined by agar gel precipitation(AGP),hemagglutination inhibitory(HI) test and reverse transcription-polymerase chain reaction(RT-PCR).
对40份盲样采用琼脂扩散试验(AGP)、血凝抑制试验(H I)和反转录聚合酶链反应(RT-PCR)进行了检测。
3)  agar gel precipitation(AGP)
琼脂扩散试验
1.
The antigen and antibody of Marek s disease virus were detected with agar gel precipitation(AGP).
对某养殖场送检的8只病死鸡,用琼脂扩散试验检出马立克氏病毒抗原阳性率为62。
2.
Agar gel precipitation(AGP) showed that two strains appeared positive reactions with BVDV Oregon C_ 24;and could be neutralized by BVDV standard positive serum,with the neutralization indices of 10 3.
51;琼脂扩散试验表明 ,2株病毒均能与牛病毒性腹泻病毒 (BVDV)OregonC2 4 标准阳性血清反应 ,出现沉淀线 ;中和试验表明 ,2株病毒均能被BVDV标准阳性血清中和 ,中和指数分别为 10 3。
4)  agar gel precipitation
琼脂扩散试验
1.
This antigen can react with positive serum of gosling plague to form precipitation lines in agar gel precipitation.
取小鹅瘟病毒经尿囊腔接种12日龄非免疫鸭胚进行传代增殖,收集尿囊液后采用聚乙二醇沉淀法制备小鹅瘟病毒沉淀抗原,经琼脂扩散试验检测,结果表明:该抗原能够与小鹅瘟阳性血清出现沉淀线,而且与小鹅瘟标准抗原的沉淀线相吻合,与其他常见的禽病血清不发生交叉沉淀反应。
5)  Agar diffusion test
琼脂扩散试验
1.
Agar diffusion tests involving the use of bacteria suspension of Vibrio Metschnihovii proceeded by formalin,boiling,acetic acid and alcohol as suspension antigen and precipitate antigen prove that positive rate of Vibrio Metschnihovii is higher via both suspension antigens of formalin and acetic acid.
采用福尔马林、煮沸、醋酸和酒精四种方法处理鸡梅氏弧菌菌悬液,分别制备上清和沉淀抗原,通过琼脂扩散试验确证福尔马林和醋酸两种方法制备的上清抗原与鸡梅氏弧菌阳性血清检出率最高,而不同处理方法制备的琼扩抗原对鸡白痢阳性血清、鸡霍乱阳性血清及鸡败血支原体阳性血清均无交叉反应。
6)  agar gel diffusion test
琼脂扩散试验
1.
Agar gel diffusion test was studied with K 99 and F 41 piliantigens extracted by heat treatment in PBS containing 2 M urea.
用含2mol/L尿素的磷酸盐缓冲液以热处理方法提取的K99和F41菌毛作为抗原,对琼脂扩散试验方法进行研究。
补充资料:铅寿扩孔试验
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CAS号:

性质:测定炸药做功能力的一种方法。系将10g炸药置于一圆柱形铅寿中央的孔中(铅寿直径及高均为200mm,孔径25mm,深125mm),引爆炸药后,爆轰产物将孔扩张为梨形,测量孔的扩张体积,以此衡量做功能力。此法简单易行,为欧洲国际炸药测试方法标准化委员会定为工业炸药的标准测试方法。常用单质炸药的铅寿扩孔值为280~550ml。

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