1) RT-nested PCR
逆转录套式PCR
1.
Development of RT-nested PCR for detection of antisense RNA recombinant retrovirus;
反义RNA重组逆转录病毒逆转录套式PCR检测方法的建立
2.
Detection of Newcastle Disease Virus by RT-nested PCR;
应用逆转录套式PCR检测新城疫病毒核酸
2) Nested reverse teranscriptase-polymerase chain resction
巢式转逆转录 PCR
3) reverse transcription-nested PCR(RT-nPCR)
逆转录巢式PCR法
4) nested reverse transcriptase polymerase chain reaction
巢式逆转录PCR
5) RT-nested-PCR
反转录套式PCR
1.
Isolated from some sick pigeons,a virus named PSH and proved to be ty pical coronavirus by injection of SPF chicken embryos,observing by electronscope ,animal regress test and RT-nested-PCR test.
从有呼吸道症状、有胰脏充出血、肺脏肉样病变的肉鸽中,通过鸡胚接种、电镜观察和反转录套式PCR分析,证实分离的病毒为冠状病毒;经过动物试验,鸡传染性支气管炎标准毒株I(BVM41)不引起试验鸽感染,而本次分离株对试验鸽具有一定的致病性,表明该分离株与IBV有较大差异,暂命名为鸽冠状病毒P(SH)。
6) RT-PCR
逆转录PCR
1.
Cloning and Sequencing of Tumor Suppressor Gene LKB1/STK11 Associated with Peutz-Jeghers Syndrome by RT-PCR;
PJ综合症相关抑癌基因LKB1/STK11的逆转录PCR法克隆及序列分析
2.
Methods Expression of PLC-epsilon in bladder tissues of 27 patients with TCCB and 12 normal bladder tissues was detected by RT-PCR,and its relationship with clinicopathological characteristics of tumor was analyzed.
方法采用逆转录PCR(RT-PCR)方法检测27例膀胱移行细胞癌和12例正常膀胱对照组中PLCε mRNA表达,分析其与膀胱病理学特征的关系。
3.
Me thods Total RNA was isolated from rat brain tissue, and the coding region of DNA polymerase β was amplified by reverse transcriptase - polymerase chain reaction (RT-PCR).
方法提取大鼠脑组织总RNA,用逆转录PCR扩增DNA多聚酶β(POLB)的编码区,与T载体连接,作DNA测序后,酶切POLB基因并连入腺病毒穿梭质粒pAdTrack-CMV,电转至含腺病毒pAdeasy骨架质粒的大肠杆菌BJ5183。
补充资料：嵌套式PCR
分子式：
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条