1) Reverse transcription-PCR (RT-PCR)
逆转录-PCR(RT-PCR)
2) RT-PCR
逆转录PCR(RT-PCR)
1.
RT-PCR was employed to observe the expression of VEGF-C among the intratumoral tissues and the peritumoral tissues and normal tissues.
选取30例喉癌组织,用逆转录PCR(RT-PCR)法研究VEGF-CmRNA在喉癌瘤内、瘤周及正常组织中的表达。
3) Reverse transcriptase-Polymerase chain reaction (RT-PCR)
逆转录酶PCR(RT-PCR)
4) RT-PCR
逆转录PCR
1.
Cloning and Sequencing of Tumor Suppressor Gene LKB1/STK11 Associated with Peutz-Jeghers Syndrome by RT-PCR;
PJ综合症相关抑癌基因LKB1/STK11的逆转录PCR法克隆及序列分析
2.
Methods Expression of PLC-epsilon in bladder tissues of 27 patients with TCCB and 12 normal bladder tissues was detected by RT-PCR,and its relationship with clinicopathological characteristics of tumor was analyzed.
方法采用逆转录PCR(RT-PCR)方法检测27例膀胱移行细胞癌和12例正常膀胱对照组中PLCε mRNA表达,分析其与膀胱病理学特征的关系。
3.
Me thods Total RNA was isolated from rat brain tissue, and the coding region of DNA polymerase β was amplified by reverse transcriptase - polymerase chain reaction (RT-PCR).
方法提取大鼠脑组织总RNA,用逆转录PCR扩增DNA多聚酶β(POLB)的编码区,与T载体连接,作DNA测序后,酶切POLB基因并连入腺病毒穿梭质粒pAdTrack-CMV,电转至含腺病毒pAdeasy骨架质粒的大肠杆菌BJ5183。
5) reverse transcription polymerase chain reaction
逆转录PCR
1.
Methods The expression of SSTR subtype 1～5 mRNA was determined by reverse transcription polymerase chain reaction method in 30 cases of pancreatic cancer and in 12 cases of normal pancreas tissues.
方法应用逆转录PCR(RT-PCR)检测30例胰腺癌组织和12例正常胰腺组织生长抑素受体亚型mRNA的表达。
6) RT-PCR
逆转录-PCR
1.
The expression of multidrug resistance(mdr 1) gene was tested by RT-PCR and Western blot.
方法采用MTT法检测X射线照射后CNE-1细胞对顺铂(cD-DP)的耐药性;采用逆转录-PCR(RT-PCR)和蛋白免疫印迹(W estern b lot)方法,检测照射后CNE-1细胞mdrl基因的表达。
补充资料：inverse PCR
分子式：
CAS号：
性质：一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA，得到某一含有已知序列的片段，将其自身连接环化，使该已知DNA两端的DNA序列连在一起，应用与已知DNA序列两末端同源的寡核苷酸为引物，可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反，故名。该法已有人用于染色体步读法(chromosome walking)。
CAS号：
性质：一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA，得到某一含有已知序列的片段，将其自身连接环化，使该已知DNA两端的DNA序列连在一起，应用与已知DNA序列两末端同源的寡核苷酸为引物，可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反，故名。该法已有人用于染色体步读法(chromosome walking)。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条