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1)  full-length cDNA
全长cDNA序列
2)  The complete nucleotide sequences
全长序列
1.
The complete nucleotide sequences of RNA1-4 of two isolates of rice stripe virus (RSV), isolated from Chuxiong (CX), Yunnan Province, and Hongze (HZ), Jiangsu Province, were determined.
与已报道的日本T分离物全长序列(17 145 nts)相比较,CX分离物变异较大。
3)  4.5S snRNA cDNA
4.5SsnRNA cDNA序列
4)  cDNA sequence
cDNA序列
1.
Methods By using bioinformatics,the cDNA sequence encoding Sj arginase was analyzed,including searching the ORF,translating the nucleotide to protein sequence,similarity searches and secondary structure predication.
目的 对获得的日本血吸虫 (Schistosomajaponicum ,Sj)精氨酸酶基因cDNA序列进行二级结构预测。
2.
After being aligned with the related cDNA sequences of Gallus gallus,Rattus norveg.
根据GenBank中鸡BMP4基因序列设计1对特异性引物,从12胚龄皖西白鹅背部皮肤中提取总RNA作为模板,采用RT-PCR技术首次扩增出鹅BMP4基因的部分cDNA序列。
3.
Accroding to the reported cDNA sequence of vitellogenin in other silk insects,the specific primers were designed.
从柳蚕雌蛹脂肪体中提取总RNA,根据已经解析出的其它泌丝昆虫的卵黄原蛋白cDNA序列设计特异性引物,对柳蚕卵黄原蛋白cDNA3′端进行RACE(rapid amplification ofcDNA ends)扩增,经克隆和测序得到了一条1 072 bp的cDNA片段,该序列与柞蚕(Antheraea pernyi)、天蚕(An-theraea yamamai)、野桑蚕(Bombyxmandarina)、家蚕(Bombyxmori)、樗蚕(Samia cynthia pryeri)、蓖麻蚕(Philosamiacynthia ricini)、樟蚕(Saturnia japonica)相应序列的同源性分别为82。
5)  full-length cDNA
全长cDNA
1.
A Full-length cDNA Library Construction of Drought Stress Erianthus arundinaceus;
斑茅抗旱全长cDNA文库的构建
2.
Amplification of full-length cDNA of Japanese encephalitis virus WHe strain by long RT-PCR;
日本脑炎病毒WHe株全长cDNA的长链RT-PCR法的扩增
3.
Construction of the genomic full-length cDNA of foot-and-mouth disease virus WFL strain;
口蹄疫病毒WFL株基因组全长cDNA的克隆及序列分析
6)  Full length cDNA
全长cDNA
1.
Objective:To establish fusion PCR for amplification of the full length cDNA of dengue virus type 2.
目的 :建立扩增登革 2型病毒基因组全长cDNA的融合PCR法 ,为深入探讨病毒基因组的结构与功能提供有效的技术途径。
2.
A full length cDNA of a β-1,3-glucanase gene was cloned in wheat leaves which were infected by Puccinia striiformis in incompatible interaction.
【目的】研究小麦抗条锈菌的分子机制,在受条锈菌侵染的非亲和组合小麦叶片中克隆一个新的β-1,3-葡聚糖酶基因全长cDNA。
3.
The full length cDNA of actin gene(accession number: EU100952),which is crucial to the growth and development of Bursaphelenchus xylophilus,is cloned by the method of in sillcon cloning and RACE in the paper.
借助电子克隆和RACE方法,克隆了松材线虫生长发育关键基因actin的全长cDNA序列(登陆号:EU100952)。
补充资料:Alu序列
分子式:
CAS号:

性质:又称Alu家族。是散在分布于哺乳动物基因组中的、含量最大的中等重复序列。由于序列中含有限制性内切酶Alu I的切点(AG↓CT)而被命名。有种族特异性,但同源性很高。人Alu序列占人基因组3%~6%,重复30万~50万次,平均每5kb就有一个。人Alu序列长300bp(碱基对),由两个130bp的重复序列间隔——31bp的插入序列组成,两侧翼各有一与转位因子序列相似的7~21bp正向重复序列,表明Alu序列是一种可转移的成分。由于Alu序列有种族特异性,克隆人基因时,用人Alu序列制备的探针可鉴定人基因的存在。发现在hn RNA中Alu序列较多而在mRNA中极少,说明其可与邻近基因一起转录,然后在RNA加工过程中切除。

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