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1)  Reverse Northern Dot_Blot
反式NorthernDot-Blot
1.
Total 79 positive clones were identified from screening of the subtracted library by PCR and Reverse Northern Dot_Blot, part of them were further verified by RT_PCR and Northern Blot.
采用巴西橡胶树常割树胶乳的Poly(A+)RNA为Tester,叶片Poly(A+)RNA为Driver,通过抑制消减杂交法(suppressivesubtractivehybridization,SSH)构建了一个胶乳特异表达基因差减文库,通过菌落PCR及反式NorthernDot-Blot筛选鉴定阳性差异片段,获得79个胶乳特异表达阳性克隆,部分阳性克隆还通过NorthernBlot杂交及RT-PCR进一步验证。
2)  reverse Northern blotting
反向Northern blot
3)  Reverse-Nothern blot
反向 Northern blot杂交
4)  inverse Northern Blot hybridization
反向Northern Blot杂交
5)  Northern blotting
Northern blot
1.
Tissue expression of the long type peptidoglycan recognition protein of mouse(mPGRP-L) was studied by RT-PCR,Northern blotting and immunohistochemistry staining.
采用RT-PCR、Northern blot以及免疫组织化学技术研究小鼠长型肽聚糖识别蛋白(mPGRP-L)在体内的表达情况。
6)  Western blot
Westem blot
补充资料:反式,反式-1,3-丁二烯-1,4-二羧酸
分子式:C6H6O4
分子量:142.11
CAS号:3588-17-8

性质:熔点290°C (dec.)。沸点320°C。水溶性insoluble。

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