1) Immuno-capture nested RT-PCR
免疫捕获巢式RT-PCR
2) IC-RT-PCR
免疫捕获RT-PCR
1.
In this study,based on the previously reported viral coat protein(CP)gene sequences,specific primers for ORSV and CyMV were designed,and four methods,including ELISA,RT-PCR,nested RT-PCR and IC-RT-PCR were performed for molecular detection of the two viruses.
本研究根据病毒外壳蛋白基因设计特异性引物,应用ELISA、普通RT-PCR、巢式RT-PCR和免疫捕获RT-PCR4种方法进行了检测研究与比较。
3) Multiplex Immuno-capture RT-PCR
多联免疫捕获RT-PCR
5) Immunocapture Reverse transcription a
免疫捕获反转录聚合酶链式反应(IC-RT-PCR)
6) Nested RT-PCR
巢式RT-PCR
1.
Development of nested RT-PCR for detection animal rabies virus
动物狂犬病病毒巢式RT-PCR检测方法的建立
2.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
3.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
补充资料:免疫PCR
分子式:
CAS号:
性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。
CAS号:
性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。
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