2) high-purity plasmid DNA
高纯度质粒DNA
1.
The influential factors in the plasmid DNA extraction on the quality of the plasmid DNA was investigated,the extraction conditions of plasmid DNA were optimized and the quality-controlling standards were set in order to produce the high-purity plasmid DNA.
利用凝胶层析,亲和层析,阴离子交换层析依次去除残余在质粒DNA中的蛋白质、RNA、非超螺旋DNA以及内毒素,达到了制备高纯度质粒DNA要求,并且达到了可以在动物实验中进行转染的质量要求。
3) DNA Purification
DNA纯化
1.
Sequence based typing (SBT) becomes the best method of HLA typing because of development of DNA amplification, DNA purification and other technology from 1 990 s, SBT is gold standard of HLA gene typing on organ transplantation, human genetics and disease association.
20世纪90年代以来DNA扩增、DNA纯化和人类基因成果对HLA领域的渗透,使得碱基序列测序方法(sequencebasedtyping,SBT)成为一种可直接阅读HLA系统复杂性的基因物质的最佳技术,并在器官移植、人类遗传学、疾病关联等研究方面被认为是HLA基因分析的金标准。
2.
DNA extraction includes two steps of cell lysis and DNA purification.
DNA提取分细胞裂解和DNA纯化2步,对细胞裂解比较了珠磨匀浆法、反复冻融法、十二烷基磺酸钠(sodium dodecyl sul-fate,SDS)裂解法等7种方法;对DNA纯化比较了酚/氯仿纯化法和胶回收纯化法。
4) Crude DNA purification
粗DNA纯化
5) DNA extraction
DNA提纯
1.
Effects of DNA extraction and purification processes on the RAPD results of six Saccharinae species;
DNA提纯方法对6种甘蔗亚族植物RAPD的影响
补充资料：DNA分子花窗(沿B―DNA轴线方向看)
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条