扩增程序,reaction system,在线英语词典,英文翻译,专业英语

1) reaction system

扩增程序

To establish a stable RAPD-PCR reaction system with good polymorphism for onion,the orthogonal design was applied to some factors in the system,such as different concentrations of Taq polymerase,Mg~(2+),primer and dNTP.

为建立多态性高、稳定性好的洋葱RAPD-PCR反应体系,采用正交设计,研究了Taq酶、Mg2+、引物和dNTP 4种RAPD-PCR反应组分浓度变化对扩增结果的影响,在此基础上对模板DNA用量、扩增程序中退火温度和反应循环次数进行了筛选。

2) PCR procedure

PCR扩增程序

And PCR procedure was optimized about the factors Mg~(++), DNA density etc.

从SDS、CTAB、高盐低pH值3种方法中选择了CTAB法作为枫香适合的DNA提取方法,同时构建了枫香DNA的PCR扩增程序,并从Mg++浓度、Taq酶用量、dNTP浓度、引物浓度、DNA模板用量等方面优化了PCR扩增程序。

3) Amplification

扩增

Integration and Amplification of Alkaline Protease Gene in Bacillus licheniformis 2709;

碱性蛋白酶基因在Bacillus licheniformis 2709中的整合和扩增

Investigation of the Key Gene in 8q24 Amplifications in Esophageal Cancer;

8q24区域扩增靶基因在食管癌中的研究

Induction and amplification of dendritic cells derived from cord blood CD34~+ cellsin vitro;

脐血CD34~+细胞体外诱导分化为树突状细胞及其扩增

4) proliferation

扩增

Effect of different cytokines on the proliferation of CD_(34)~+ hematopoietic cells in cord blood*;

不同细胞因子对脐血CD_(34)~+细胞扩增的影响

HIM is the soil of orientation, proliferation, differentiation and development of HSC, of which hematopoietic stromal cell is an important component.

人脐血中是否存在造血基质细胞及其生物学特点如何，脐血基质细胞能否在体外有效扩增，国内外相关的系统性研究较少。

5) Expansion

扩增

Targeted Expansion and Regulation of Genetically Modified Cord Blood Stem/Progenitor Cells in vitro;

靶基因调控的脐血干/祖细胞体外长期扩增与调控的实验研究

The effects of bone marrow stromal cells conditioned medium combined with TPO on the expansion of mature megakaryocytes and CFU-Meg in vitro;

骨髓基质细胞条件培养液联合TPO体外扩增巨核系细胞

Selective Expansion of Human Natural Killer Cells;

选择性扩增人自然杀伤细胞的实验研究

6) PCR amplification

PCR扩增

Effects of DMSO Concentration on the PCR Amplification of GC-rich Micromonospora Genome DNA;

二甲基亚砜对高GC含量小单孢菌基因组DNA PCR扩增的影响

Molecular cloning and PCR amplification of plasmids in vitro against late blight for potato;

抗马铃薯晚疫病质粒的分子克隆及其体外PCR扩增

A PCR amplification system comprising a syringe pump sample processor, a microfluidic PCR chip and a 3-temperature-zone heating block was developed in this work.

组装了由注射泵进样系统、微流控芯片和三温区加热器组成的流动型 PCR扩增系统 ,该系统具有扩增速度快、交叉污染小、芯片可重复使用和操作方便等特点。

参考词条

RACE扩增扩增倍数扩增效率分段扩增细胞扩增扩增突变质粒扩增 DNA扩增核酸扩增祁连山褶皱带 SZ

补充资料：分子扩增

分子式：
CAS号：

性质：又称无性繁殖，克隆繁殖，分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质，即DNA大分子提取出来，在离体条件下进行切割后，取其片段，或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来，然后再导入某一受体细胞中，让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始，它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引基因扩增扩增内标扩增曲线体外扩增 RAPD扩增复合扩增

1) reaction system 扩增程序 1. To establish a stable RAPD-PCR reaction system with good polymorphism for onion,the orthogonal design was applied to some factors in the system,such as different concentrations of Taq polymerase,Mg~(2+),primer and dNTP. 为建立多态性高、稳定性好的洋葱RAPD-PCR反应体系,采用正交设计,研究了Taq酶、Mg2+、引物和dNTP 4种RAPD-PCR反应组分浓度变化对扩增结果的影响,在此基础上对模板DNA用量、扩增程序中退火温度和反应循环次数进行了筛选。 2) PCR procedure PCR扩增程序 1. And PCR procedure was optimized about the factors Mg~(++), DNA density etc. 从SDS、CTAB、高盐低pH值3种方法中选择了CTAB法作为枫香适合的DNA提取方法,同时构建了枫香DNA的PCR扩增程序,并从Mg++浓度、Taq酶用量、dNTP浓度、引物浓度、DNA模板用量等方面优化了PCR扩增程序。 3) Amplification 扩增 1. Integration and Amplification of Alkaline Protease Gene in Bacillus licheniformis 2709; 碱性蛋白酶基因在Bacillus licheniformis 2709中的整合和扩增 2. Investigation of the Key Gene in 8q24 Amplifications in Esophageal Cancer; 8q24区域扩增靶基因在食管癌中的研究 3. Induction and amplification of dendritic cells derived from cord blood CD34~+ cellsin vitro; 脐血CD34~+细胞体外诱导分化为树突状细胞及其扩增 4) proliferation 扩增 1. Effect of different cytokines on the proliferation of CD_(34)~+ hematopoietic cells in cord blood; 不同细胞因子对脐血CD_(34)~+细胞扩增的影响 2. HIM is the soil of orientation, proliferation, differentiation and development of HSC, of which hematopoietic stromal cell is an important component. 人脐血中是否存在造血基质细胞及其生物学特点如何，脐血基质细胞能否在体外有效扩增，国内外相关的系统性研究较少。 5) Expansion 扩增 1. Targeted Expansion and Regulation of Genetically Modified Cord Blood Stem/Progenitor Cells in vitro; 靶基因调控的脐血干/祖细胞体外长期扩增与调控的实验研究 2. The effects of bone marrow stromal cells conditioned medium combined with TPO on the expansion of mature megakaryocytes and CFU-Meg in vitro; 骨髓基质细胞条件培养液联合TPO体外扩增巨核系细胞 3. Selective Expansion of Human Natural Killer Cells; 选择性扩增人自然杀伤细胞的实验研究 6) PCR amplification PCR扩增 1. Effects of DMSO Concentration on the PCR Amplification of GC-rich Micromonospora Genome DNA; 二甲基亚砜对高GC含量小单孢菌基因组DNA PCR扩增的影响 2. Molecular cloning and PCR amplification of plasmids in vitro against late blight for potato; 抗马铃薯晚疫病质粒的分子克隆及其体外PCR扩增 3. A PCR amplification system comprising a syringe pump sample processor, a microfluidic PCR chip and a 3-temperature-zone heating block was developed in this work. 组装了由注射泵进样系统、微流控芯片和三温区加热器组成的流动型 PCR扩增系统 ,该系统具有扩增速度快、交叉污染小、芯片可重复使用和操作方便等特点。参考词条 RACE扩增扩增倍数扩增效率分段扩增细胞扩增扩增突变质粒扩增 DNA扩增核酸扩增祁连山褶皱带 SZ 补充资料：分子扩增分子式： CAS号：性质：又称无性繁殖，克隆繁殖，分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质，即DNA大分子提取出来，在离体条件下进行切割后，取其片段，或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来，然后再导入某一受体细胞中，让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始，它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。说明：*补充资料仅用于学习参考，请勿用于其它任何用途。
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