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1)  Amplification efficiency
扩增效率
1.
Objective: To investigate the amplification efficiency of primers on different sites of BCL2 alleles in real-time PCR reactions and the reliability of these primers in analysis of transcription activity of different alleles with vector construction and real-time PCR.
目的:采用载体构建及荧光定量PCR的方法比较BCL2等位基因不同部位PCR引物在定量分析中的扩增效率,判断其用于不同等位基因转录活性分析的可靠性。
2.
Conclusion:The amplification efficiency of primers have significant impact on quantitative PCR analysis.
结果:统计学分析表明,引物的扩增效率对定量PCR结果分析有显著影响,不同引物特异性扩增得到的样本间表达倍数有显著差异(P<0。
2)  Amplification effect
扩增效果
1.
S-genotypes of 30 apricot cultivars native to China were detected using S-allele PCR with 5 pairs of Prunus consensus primer combinations which had been published, and amplification effects were evaluated according to coefficient and efficiency of amplification in the work.
本研究选用已报道的5对蔷薇科李属通用引物组合对起源于我国的30个杏品种进行了S等位基因的PCR扩增,依据扩增系数和扩增成功率两个指标对引物的扩增效果进行了评价,利用扩增效果最好的引物组合EM-PC2consFD+EM-PC3consRD并结合田间授粉试验对其中的16个品种进行了S基因型鉴定,旨在为生产果园授粉树的合理配置提供科学依据,并为进一步探讨杏自交不亲和的分子机理提供基本资料。
3)  efficiency of pressure expanding
扩压效率
4)  plasticizing efficiency
增塑效率
1.
Effect of plastifiers on compatibility,plasticizing efficiency,mechanical properties of SPU sealants were discussed by swelling test and dynamic properties analysis.
通过溶胀实验和动态力学性能分析,讨论了增塑剂对SPU密封胶的溶胀性能、增塑效率以及力学性能的影响。
5)  Toughening efficiency
增韧效率
6)  rate of carburization
增碳效率
1.
Results from tests on carburization in steelmaking indicate that the rate of carburization for coal based carhuretant is equal to that for the asphalt based carburetant and substitution of the former for the latter which is widely used will not only have little influence on steel quality, but can also bring noticeable economical benefits to the enterprise.
通过炼钢增碳试验表明:煤基增碳剂的增碳效率与沥青基增碳剂相当,用其代替目前普遍使用的沥青基增碳剂炼钢增碳不影响钢的质量,同时可提高企业经济效益。
补充资料:分子扩增
分子式:
CAS号:

性质:又称无性繁殖,克隆繁殖,分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质,即DNA大分子提取出来,在离体条件下进行切割后,取其片段,或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来,然后再导入某一受体细胞中,让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始,它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。

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