2) anti-sense expression vector construction
反义基因表达载体构建
3) construction of sense and antisense expressed vector
正向、反向表达载体构建
5) Antisense expression vector
反义表达载体
1.
Molecular cloning of a muskmelon cDNA fragment encoding an amino-cyclopropane-1-carboxylie acid(ACC) synthase and construction of antisense expression vector;
薄皮甜瓜果实ACC合成酶cDNA克隆及反义表达载体的构建
2.
The FaEtr1 and FaErs1 cDNA were inserted into reverse orientation between the CaMV 35S promoter and Nos terminator of the expression vector pBI121,respectively,and two antisense expression vectors pBI121Etr1 and pBI121Ers1 were obtained.
在已报道的FaEtr1和FaErs1基因序列的基础上,设计特异引物,分别克隆草莓(Fragaria ananassa)乙烯受体FaEtr1基因580bp部分特异序列和FaErs1基因445bp部分特异序列,将上述2个片段分别反向插入植物表达载体pBI121的CaMV35S启动子和Nos终止子之间,构建了反义表达载体pBI121Etr1和pBI121Ers1。
3.
An antisense expression vector,named pCAM-ACO_2,of the DC-ACO gene was constructed,in which the antisense sequence was controlled by the CaMV 35S promoter.
将克隆的香石竹ACO片段反向连接到植物表达载体pCAMBIA 1301中CaMV 35S启动子的下游,构建了香石竹ACO基因的反义表达载体pCAM-ACO2,为进一步通过反义技术延长转基因香石竹的花期奠定了基础。
6) construction of expression vector
表达载体构建
1.
Construction of expression vector of nematode-resistant gene and transformation of sugarcane;
抗线虫基因表达载体构建与转化甘蔗研究初报
补充资料：反载体
见载体。
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参考词条