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1)  collection embryo
采卵
1.
Analysis of different treatment programs of washing uterus after collection embryo on donor cows of different species;
供体牛采卵后不同清宫处理方法的效果分析
2)  Oocyte retrieval rate
采卵率
3)  ovum pick up
活体采卵
1.
Ten sterile female boer goat donors were tested in this research,which were approached to breed normal offsprings by the techniques of superovulation,ovum pick up,maturation culture in vitro and fertilization in vitro of the maturated oocytes etc.
 对10只不孕不育波尔母山羊采用超数排卵、活体采卵、卵母细胞体外成熟培养以及体外受精等技术方法,探讨其是否能正常繁育后代。
2.
Bovine oocytes were collected from 5 naturally cycling high yield cows by repeated transvaginal ultrasound scanning guided puncturing of visible follicles using 2 ways ovum pick up needle.
7 k Pa 抽吸压经阴道对 5 头高产奶牛分别连续实施 7 次活体采卵,每 7 d 1 次,共采集卵子 214 个,占可见卵泡数的 53。
4)  OPU
活体采卵
1.
Follicular development and ovum pick-up(OPU) on kids following hormone treatment;
羔山羊卵泡诱导发育及活体采卵研究
2.
Study on the Technique of Ovum Pick-up (OPU) and in Vitro Fertilization (IVF) in Bovine;
牛活体采卵及体外受精技术的研究
3.
The major factors affected the Laparoscopic Ovum Pick-up (LOPU) and in vitrofertilization (IVF) technique were discussed systematically in this study in order toestablish an acceptable manipulation and production system of LOPU-IVF in our lab.
本实验针对如何提高腹腔镜进行山羊活体采卵和体外受精效果开展了多方面的研究,以便完善“活体采卵—体外受精”生产体外胚胎的技术体系,进而提高体外胚胎生产的效率。
5)  ovum pick-up
活体采卵
1.
A number of oocytes with specific genealogy can be retrieved repeatedly from living cattle by the technology of ovum pick-up(OPU),and these oocytes are used to produce embryos in vitro.
应用活体采卵技术可以从活体母牛反复采集大量系谱明确的卵母细胞,用于胚胎的体外化生产。
2.
The effect of donor cows treated with pregnant mare serum gonadotrophin(PMSG) or follicular stimulating hormone(FSH) on oocyte recovery by ovum pick-up(OPU) and in vitro embryo production was studied.
选择6头3~6岁健康荷斯坦、空怀母奶牛随机分为3组,每组2头,分别用外源激素孕马血清促性腺激素(PM SG)、促卵泡素(FSH)、空白对照处理供体奶牛,4 d后应用B型超声波导引采卵,以研究外源激素对活体采卵效率和体外生产胚胎的影响。
3.
In this study, these technology, for example, ovum pick-up, in vitro fertilization and embryo splitting, were studied.
为了对牛早期胚胎的性别进行鉴定,本试验研究了活体采卵、体外受精和胚胎切割等技术。
6)  ovum pick-up(OPU)
活体采卵
1.
Transvaginal ultrasound-guided ovum pick-up(OPU)was performed on 40 Nili-Ravi and Murrah buffaloes for consecutive 3 times each,at an interval of 3 days.
这表明,在B超引导下进行连续活体采卵,并利用卵母细胞进行体外受精生产胚胎是可行的。
补充资料:采卵


采卵


在控制超排卵药刺激下,应用阴道B超监测卵泡发育,血E2浓度测定及其他辅助方法如子宫颈黏液检查,尿LH峰测定,判定取卵时间。一般在绒毛促性腺激素注射后32~36小时取卵。现大都应用B超引导下阴道一根针取卵。在消毒情况下,转动阴道探头,探及卵巢及卵泡,穿刺针插入固定针导中,将针迅速刺入卵泡中心,负压吸引,压力为16kPa(120mmHg)。卵泡液吸出,可见卵泡缩小。从卵泡液中寻找卵冠丘复合物,如找不到,可冲洗卵泡一、二次;再进入第2个卵泡抽吸。这种取卵技术创伤性最小,不需麻醉,术前给止痛及镇静药。为了简化步骤,术前不用连续取血测E2值。阴道取卵技术是1986年以后逐步发展起来的,过去一般都采用腹腔镜取卵。
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