半套式聚合酶链反应,hemi-nested polymerase chain reaction,音标,读音,翻译,英文例句,英语词典

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1) hemi-nested polymerase chain reaction 点击朗读

半套式聚合酶链反应

A hemi-nested polymerase chain reaction (PCR) assay was established to detect TTV DNA in 40 pairs of sera from mother and paired cord sera, and the PCR-amplified products from a mother and her infant was sequenced and the sequences were analyzed with the computer programs.

为了解TT病毒（TTV）在我国母婴间传播的可能及分子病毒学依据,合成引物（引物1:5’-ACAGACAGAGGAGAAGGCAACATG-3’;引物2:5’CTGGCATTTTACCATTTCCAAAGTT-3’;引物3:5’-GGCAACATGTTATGGATAGACTGG-3＇）,采用半套式聚合酶链反应（hemi-nested PCR）方法,检测了40对母、婴配对血消中的TTV　DNA,并对TTV　DNA均阳性的一对母婴配对血清中PCR产物进行测序和序列分析。

2) nestedPCR 点击朗读

嵌套式聚合酶链式反应

To develop a nestedpolymerase chain reaction (nestedPCR) for detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease, 2 pairs of primers, BKDF and BDKR, BKDR2 and BKDF2, were designed for amplification of 501 bp and 314 bp DNA fragments of the sequence coding the 57 kDa cell surface protein of R.

描述了用嵌套式聚合酶链式反应(nested PCR)快速检测鲑鱼细菌性肾病病原鲑鱼肾杆菌的方法。

3) Nested-PCR 点击朗读

套式聚合酶链式反应

The mutatiom of p53 gene were observed in 30 cases of colorectal cancer patients by Nested-PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism) analysis,and examined on the exon 5～8 mainly.

用套式聚合酶链式反应—单链构象多态分析法对３０例结肠癌的ｐ５３基因突变进行了观察，检测了第５～８四个外显子，发现２１例有ｐ５３基因突变（７０％）。

更多例句>>

4) nested-PCR 点击朗读

套式聚合酶链反应

例句>>

5) Nested polymerase chain reaction 点击朗读

套式聚合酶链反应

Genetic polymorphism of apolipoprotein CII gene dinucleotide tandem repeats (TG)n(AG)m and (AG)m was analysed by using nested polymerase chain reaction and polyacrylamide gel electrophoresis.

采用套式聚合酶链反应结合变性聚丙烯酰胺凝胶电泳和银染技术 ,并构建载脂蛋白CII (ApoCII)基因二核苷酸串联重复序列 (TG)n(AG)m及 (AG)m序列等位基因梯阶标准 ;检测正常汉族人群基因型和等位基因频率分布 ,检出 36种 (TG)n(AG)m序列基因型、12种等位基因。

The methods for detection of Ct by major outer membrane protein （MOMP）gene (ompl) nested polymerase chain reaction (ompl-nPCR), DNA purification and DNA sequencing were studied and compared.

目的探讨沙眼衣原体（Ct）套式聚合酶链反应（nPCR）、DNA纯化、测序的检测方法；首次建立Ct上游引物5′末端地高辛(Dig)标记裂解酶片段长度多态性（CFLP）分型方法并探索和优化Ct主要外膜蛋白（MOMP）基因（ompl）第Ⅳ可变区（VDⅣ）变温CFLP条件;了解重庆地区近年来临产孕妇Ct感染和母婴垂直传播状况及常见基因型。

更多例句>>

6) nested PCR 点击朗读

套式聚合酶链反应

例句>>

补充资料：聚合酶链反应

聚合酶链反应
polymerase chain reaction,PCR

是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

反转录-复合套式聚合酶链式反应反转录套式聚合酶链式反应聚合酶链式反应荧光定量套式逆转录酶聚合酶链反应反转录-嵌套式聚合酶链反应反转录套式聚合酶链反应

半巢式聚合酶链反应复合反转录-套式聚合酶链式反应

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	您的位置：首页 -> 词典 -> 半套式聚合酶链反应 1) hemi-nested polymerase chain reaction 半套式聚合酶链反应 1. A hemi-nested polymerase chain reaction (PCR) assay was established to detect TTV DNA in 40 pairs of sera from mother and paired cord sera, and the PCR-amplified products from a mother and her infant was sequenced and the sequences were analyzed with the computer programs. 为了解TT病毒（TTV）在我国母婴间传播的可能及分子病毒学依据,合成引物（引物1:5’-ACAGACAGAGGAGAAGGCAACATG-3’;引物2:5’CTGGCATTTTACCATTTCCAAAGTT-3’;引物3:5’-GGCAACATGTTATGGATAGACTGG-3＇）,采用半套式聚合酶链反应（hemi-nested PCR）方法,检测了40对母、婴配对血消中的TTV　DNA,并对TTV　DNA均阳性的一对母婴配对血清中PCR产物进行测序和序列分析。 2) nestedPCR 嵌套式聚合酶链式反应 1. To develop a nestedpolymerase chain reaction (nestedPCR) for detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease, 2 pairs of primers, BKDF and BDKR, BKDR2 and BKDF2, were designed for amplification of 501 bp and 314 bp DNA fragments of the sequence coding the 57 kDa cell surface protein of R. 描述了用嵌套式聚合酶链式反应(nested PCR)快速检测鲑鱼细菌性肾病病原鲑鱼肾杆菌的方法。 3) Nested-PCR 套式聚合酶链式反应 1. The mutatiom of p53 gene were observed in 30 cases of colorectal cancer patients by Nested-PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism) analysis,and examined on the exon 5～8 mainly. 用套式聚合酶链式反应—单链构象多态分析法对３０例结肠癌的ｐ５３基因突变进行了观察，检测了第５～８四个外显子，发现２１例有ｐ５３基因突变（７０％）。更多例句>> 4) nested-PCR 套式聚合酶链反应例句>> 5) Nested polymerase chain reaction 套式聚合酶链反应 1. Genetic polymorphism of apolipoprotein CII gene dinucleotide tandem repeats (TG)n(AG)m and (AG)m was analysed by using nested polymerase chain reaction and polyacrylamide gel electrophoresis. 采用套式聚合酶链反应结合变性聚丙烯酰胺凝胶电泳和银染技术 ,并构建载脂蛋白CII (ApoCII)基因二核苷酸串联重复序列 (TG)n(AG)m及 (AG)m序列等位基因梯阶标准 ;检测正常汉族人群基因型和等位基因频率分布 ,检出 36种 (TG)n(AG)m序列基因型、12种等位基因。 2. The methods for detection of Ct by major outer membrane protein （MOMP）gene (ompl) nested polymerase chain reaction (ompl-nPCR), DNA purification and DNA sequencing were studied and compared. 目的探讨沙眼衣原体（Ct）套式聚合酶链反应（nPCR）、DNA纯化、测序的检测方法；首次建立Ct上游引物5′末端地高辛(Dig)标记裂解酶片段长度多态性（CFLP）分型方法并探索和优化Ct主要外膜蛋白（MOMP）基因（ompl）第Ⅳ可变区（VDⅣ）变温CFLP条件;了解重庆地区近年来临产孕妇Ct感染和母婴垂直传播状况及常见基因型。更多例句>> 6) nested PCR 套式聚合酶链反应例句>> 补充资料：聚合酶链反应聚合酶链反应 polymerase chain reaction,PCR 是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。 说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条反转录-复合套式聚合酶链式反应反转录套式聚合酶链式反应聚合酶链式反应荧光定量套式逆转录酶聚合酶链反应反转录-嵌套式聚合酶链反应反转录套式聚合酶链反应

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