1) reverse transcription-nested polymerase chain reaction (RT-nested PCR)
反转录-嵌套式聚合酶链反应
2) multiplex reverse transcription-nested polymerase chain reaction
复合反转录-套式聚合酶链式反应
1.
A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus;
检测和鉴别犬瘟热病毒强、弱毒株的复合反转录-套式聚合酶链式反应(RT-nPCR)的建立及初步应用
3) Reverse transcription-multiplex nested polymerase chain reaction
反转录-复合套式聚合酶链式反应
4) NRT-PCR
嵌套式反转录聚合酶键反应
5) nestedPCR
嵌套式聚合酶链式反应
1.
To develop a nestedpolymerase chain reaction (nestedPCR) for detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease, 2 pairs of primers, BKDF and BDKR, BKDR2 and BKDF2, were designed for amplification of 501 bp and 314 bp DNA fragments of the sequence coding the 57 kDa cell surface protein of R.
描述了用嵌套式聚合酶链式反应(nested PCR)快速检测鲑鱼细菌性肾病病原鲑鱼肾杆菌的方法。
6) Reverse transcription nested polymerase chain reaction (RT-nested PCR)
反转录套式聚合酶链式反应
补充资料:聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条