1) indirect immuno-fluorescent staining
间接免疫荧光染色
1.
Detection of Erwinia amylovora by indirect immuno-fluorescent staining and co-agglutination methods;
利用间接免疫荧光染色和协同凝集技术检测梨火疫病菌
2.
The method of indirect immuno-fluorescent staining(IFA) was applied to investigate the invading process and distribution of DEV.
用鸭病毒性肠炎病毒(DEV)CHv强毒株感染成年鸭复制鸭病毒性肠炎急性病例,分别于接种后不同时间,取心、肝、脾、肺、肾、胸腺、食道、十二指肠、胰腺、法氏囊和脑组织,制作切片,应用间接免疫荧光染色法(IFA)检测DEV在鸭体内的侵染过程和分布规律。
2) immunofluorescence
[英][i'mju:nəu,fluə'resns] [美][,ɪmjəno,flʊə'rɛsəns]
间接免疫荧光染色
1.
The obtained cells were observed using transmission electron microscopy(TEM),detected with alizarin red staining and Gram staining,and identified by indirect immunofluorescence staining .
运用透射电镜、钙染色和革兰染色等手段观察其形态特征,采用单克隆抗体间接免疫荧光染色进行细菌鉴定。
3) indirect immuno-fluorescent staining(IIFS)
间接免疫荧光染色技术
4) indirect immunofluorescent antibody metheod
间接免疫荧光抗体染色法
1.
The replication of two strains of NIBV in Chicken Embryo Kidney Cells(CEK) were verified by indirect immunofluorescent antibody metheod ,by immunoelectronic microscope technology,by Gimsa stain metho.
将经过系统鉴定的 2株鸡肾型传染性支气管炎病毒 (NIBV)在鸡胚肾细胞上盲传 7代后 ,均可不同程度的产生特征性细胞病变效应 (CPE) ,表现为细胞膜界限消失及多核巨细胞的形成 ,并通过间接免疫荧光抗体染色法 ,免疫复合物电镜观察及姬姆萨染色法对 CEK适应毒进行鉴定 ,结果表明 2株 NIBV鸡胚适应毒经多次传代后均适应于 CEK细
5) immunofluorescence staining
免疫荧光染色
1.
Methods Cells were harvested at 0 day,the 3rd day and the 7th day after BGSCs differentiation,then the ratio of Hes-1 positive cells were counted by flow cytometry,and the expression intensity and cell types were detected by laser confocal microscopy after immunofluorescence staining with Hes-1 and GFAP,MAP2 or MBP.
方法对体外培养的人脑胶质瘤干细胞进行诱导分化,于未分化、分化第3、7d收集标本,行Hes-1蛋白免疫荧光染色后流式细胞术检测标本中阳性细胞比例;Hes-1分别与CD133、GFAP、MAP2、MBP蛋白双标免疫荧光染色后,激光共聚焦显微镜下观察Hes-1蛋白表达强度及细胞类型的变化。
2.
The nNOS neurons were detected by immunofluorescence staining.
用免疫荧光染色、RT-PCR和Western blot方法对nNOS神经元数量、nNOS基因和蛋白的表达量进行检测。
3.
The aim of this experiment was to study the effective method of fixation and penetration before immunofluorescence staining in oocytes and early embryos to raise the effect of immunofluorescence staining in them.
本实验旨在探索有效的染色前固定和渗透卵母细胞及早期胚胎的方法,以提高小鼠卵母细胞和早期胚胎免疫荧光染色的效果;同时检测RNA聚合酶Ⅱ(PolⅡ)在小鼠卵母细胞和早期胚胎的表达,探讨PolⅡ在卵母细胞和早期胚胎发育中的作用。
6) Immunofluorescent staining
免疫荧光染色
1.
The purpose of this article is to explore The Organization of cochlear immunofluorescent staining and laser scanning confocal microscope technology applications.
本文目的是探讨耳蜗组织免疫荧光染色及激光共聚焦显微镜技术的应用。
2.
Results Immunofluorescent staining showed predominantly P2X7 and P2X2 receptor expression in OHCs and the distribution of P2X7 receptor overlaped with prestin expression.
结果免疫荧光染色结果:在外毛细胞上仅见P2X2和P2X7两种亚型表达,且P2X7的分布与外毛细胞电运动的基础Prestin蛋白的分布相重合。
补充资料：精子间接免疫荧光法
精子间接免疫荧光法
诊法。免疫学检查方法之 一。用于不育症检查。①以志愿供者的精液02ml加入10～12ml生理盐水中离心，取其精子沉淀部分再置入05ml生理盐水内。②取一滴作玻璃涂片，并在空气内晾干30分钟，用纯甲醇固定30分钟，再晾干后置入pH为74的磷酸缓冲液内15分钟。③被测血清滴于上述制备的玻片上，室温下孵化30～60分钟后，用磷酸缓冲液漂洗3次，每次10分钟。④玻片上滴注 附有抗人体免疫球蛋白抗血清的异硫氰酸荧光素盐酸盐，室温下孵化30～60分钟，孵化后用磷酸缓冲液漂洗3次，每次10分钟。⑤荧光显微镜下观察结果。利用血清内的精子抗体，可使精子不同部位呈现不同免疫荧光素反应，通过与正常血清对比研究，以判断精子抗体的存在情况。
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参考词条