1) Indirect immunofluorescence
间接免疫荧光法
1.
Application of indirect immunofluorescence(IFL) in the detect of antibody to eberthella typhi and bacillus paratyphosus;
间接免疫荧光法(IFL)测抗伤寒、副伤寒沙门菌IgM抗体
2.
Methods: The ANA was detected with indirect immunofluorescence(IIF) and protein chip respectively,and the other six autoantibodies including anti-dsDNA,anti-Ro-60/SSA,anti-Ro-52/SSA,anti-SSB,anti-Jo-1,and anti-phospholipid IgG were simultaneously detected with ELISA and protein chip technology,and numbers of the both positive,both negative and the si.
方法用酶联免疫吸附试验(EL ISA)、蛋白芯片法检测抗dsDNA、抗R o-60/SSA、抗R o-52/SSA、抗SSB、抗Jo-1、抗磷脂IgG抗体,用间接免疫荧光法(IIF)、蛋白芯片法检测抗核抗体(ANA),分别统计两种方法检测均阳性、均阴性和单阳性的标本数目,并进行评价。
3.
METHODS: Balb/c mice was immunized with pig cardiac myosin and complete freund ,s adjuvant(CFA), β 1 integrin on myocardium were analyzed by laser confocal microscopy and indirect immunofluorescence at the day 14,30,60 after the first immunization.
方法:猪肌凝蛋白皮下注射免疫Balb/c小鼠,建立慢性自身免疫性心肌炎模型,于初次免疫后14、30和60d对心肌组织进行病理学检查,同时用激光共聚焦技术和间接免疫荧光法检测整合素β1在心肌的表达变化。
2) Indirect immunofluorescence assay
间接免疫荧光法
1.
Then,its transcription,expression and localization were determined by real-time RT-PCR,Western-blotting and indirect immunofluorescence assay.
1-UL51,通过脂质体介导将其转入COS-7细胞;应用实时荧光RT-PCR、Western-blotting和间接免疫荧光法检测UL51基因在COS-7细胞中的转录、表达和定位情况。
3) Indirect immunofluorescent assay
间接免疫荧光法
1.
Methods Indirect immunofluorescent assay(IFA) was developed to detect the sera from suspicious Dengue patients in 2003-2004.
方法用间接免疫荧光法(IFA)检测浙江省2003～2004年疑似登革热病人血清。
4) indirect immunofluorescence(IIF)
间接免疫荧光法
1.
Objective To found the indirect immunofluorescence(IIF) of detecting immunoglobulin binding protein(Bip) antibody with Hela cells as antigenic sources and to provide the means for exploring the diagnostic value of Bip antibody in patients with rheumatoid arthritis(RA).
目的建立抗免疫球蛋白结合蛋白(Bip)抗体检测的间接免疫荧光法(IIF),为探讨其在类风湿关节炎(RA)中的意义做出方法学准备。
2.
Antinuclear antibodies(ANA)、antimitochondrial antibody(AMA) and anti-smooth muscle antibody(SMA) were tested by indirect immunofluorescence(IIF);Antibodies to soluble liver antigen/liver pancreas(SLA/LP)、l.
采用间接免疫荧光法(IIF)检测各组抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA);采用免疫印迹法检测抗可溶性肝抗原/肝胰抗原(SLA/LP)抗体、抗肝肾微粒体抗体I型(LKM-1)、抗肝细胞浆抗体1型(LC-1)、抗线粒体抗体Ⅱ型(AMA-M2),并分析各组阳性率。
5) IFAT
间接免疫荧光法
1.
Objective:To study the application of indirect immunofluorescent antibody technique in detecting rabies antibody IgG(refers to IFAT as follows).
间接免疫荧光技术与ELISA同时检测狂犬病抗体,间接免疫荧光法阳性率为92。
2.
[Objective] To study the application of indirect immunofluorescent antibody technique to detect rabies antibody IgG (refers to " IFAT" as follows) .
间接免疫荧光技术与ELISA同时检测,间接免疫荧光法阳性率为82。
6) IFA
间接免疫荧光抗体法
1.
Methods:Over a 5-year period(2000-2004),the serum antibodies measurement of against Lyme borreliosis with the serological tests:a sensitive IFA as the first step,followed by WB(IgM and IgG)in 2 167 people s serum specimen of Xinjiang natural foci compared with random sampling 1 443 people from out-patient clinic and hospital patients in country and ci.
方法:2000至2004年采用间接免疫荧光抗体法(IFA)、蛋白印迹法(WB),对业已证实的新疆自然疫源地区2167人份的血清标本进行抗LD螺旋体抗体(IgM、IgG)检测;采用IFA、WB、ELISA试验对来自21个县、市的门诊与住院患者,随机抽样1443人份血清标本进行抗LD螺旋体抗体(IgM、IgG)检测作对照;共计3610份血清标本。
2.
Methods:The sera of 123 suspected cases and part of spinal fluids were collected to detect the specific antibodies (IgM,IgG) to Borrdlia burgdorferi by enzyme linked immunosorbent assay (ELISA),indirect flouorescent antibody (IFA) test,and Western blot (WB).
方法:应用间接免疫荧光抗体法(IFA)、酶联免疫吸附试验(ELISA)、蛋白印迹法(WB)3种方法检测新疆123例疑似莱姆病患者血清及部分脑脊液抗伯氏疏螺旋体IgM、IgG抗体,其中3例PCR法检测尿液,结合相应的影像学、电生理检查诊断莱姆病。
补充资料：精子间接免疫荧光法
精子间接免疫荧光法
诊法。免疫学检查方法之 一。用于不育症检查。①以志愿供者的精液02ml加入10～12ml生理盐水中离心，取其精子沉淀部分再置入05ml生理盐水内。②取一滴作玻璃涂片，并在空气内晾干30分钟，用纯甲醇固定30分钟，再晾干后置入pH为74的磷酸缓冲液内15分钟。③被测血清滴于上述制备的玻片上，室温下孵化30～60分钟后，用磷酸缓冲液漂洗3次，每次10分钟。④玻片上滴注 附有抗人体免疫球蛋白抗血清的异硫氰酸荧光素盐酸盐，室温下孵化30～60分钟，孵化后用磷酸缓冲液漂洗3次，每次10分钟。⑤荧光显微镜下观察结果。利用血清内的精子抗体，可使精子不同部位呈现不同免疫荧光素反应，通过与正常血清对比研究，以判断精子抗体的存在情况。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条