1)  Ci-ELISA
间接竞争酶联免疫吸附测定法
1.
The Ci-ELISA method for the detection of difloxacin were established with the polyclonal antibodies against difloxacin.
以合成的二氟沙星(DIF)人工抗原为基础,制备出了高效价抗DIF血清(1∶29×104),并建立了DIF间接竞争酶联免疫吸附测定法(Ci-ELISA法)。
2)  indirect
间接
1.
Comparison between methods for measuring oxygen consumption in patients after cardiac surgery: the reverse Fick method versus indirect calorimetry;
间接测热法和反向Fick氏法测定心脏手术病人术后氧耗量的比较
2.
Structural Decomposition Analysis of China s Indirect Household Energy Consumption;
中国居民间接生活能源消费的结构分解分析
3.
Analysis of Causes of the Current Indirect Social Problem of School P.E.in Our Country;
当前我国学校体育间接社会问题的成因分析
3)  Indirect determination
间接测定
1.
Indirect Determination of Cystine by Flame Atomic Absorption Spectrometry;
原子吸收光谱间接测定胱氨酸的研究
2.
Indirect determination of trace iodine by hydride generation-atomic fluorescence spectrometry;
氢化物发生-原子荧光光谱法间接测定痕量碘的研究
3.
Indirect Determination of Trace Sulfate Ion in Sea Water by Graphite Furnace Atomic Absorption Spectrometry;
石墨炉原子吸收光谱法间接测定海水中的微量硫酸根
4)  indirect method
间接法
1.
Nanocrystals Y2O2S:Eu3+(molar ratio of Eu3+ /Y3+ was 2%) were prepared by indirect method.
用间接法制备纳米级红色荧光粉Y2O2S:Eu3+(物质的量之比为Eu3+/Y3+=0。
2.
The indirect method is prior to the direct method in its safety and equipment,while the direct method is prior to the indirect method in production efficiency and energy consumption.
文章比较了两种典型的硝酸甲胺制造工艺的安全及经济技术特征,认为从安全性及对设备的要求方面分析,间接法优于直接法;从生产效率及能耗方面比较,直接法优于间接法。
3.
Some dispute problems about the content of coal bed gas(methane) predicted by indirect method,including the existing status of the methane,the adsorption isotherm models for coal bed gas and their relationships,the free methane state in coal seam and the parameters for its capacity calculation,were discussed and analysed.
对间接法预测煤层气(甲烷)含量有关的煤层气在煤中赋存状态、煤层气在煤中吸附模型及其相互之间的联系、煤层中游离煤层气及其含量相关计算所需的参数进行了分析讨论,并提出了相应的建议。
5)  indirect spot welding
间接点焊
1.
Effect of processinig parameters on sheet-tube indirect spot welding process;
工艺参数对板管间接点焊过程的影响
6)  indirect monitoring
间接监控
1.
The temperature inspecting and measurement is an important part of parameters monitoring of operating environment, according to the characteristics of temperature field variation of chain grate, an indirect monitoring system based on indirect monitoring technology and the dead-time and forward feedback PID control algorithm is designed in this paper.
链篦机篦床温度场监控是球团生产线链篦机运行环境参数监控的主要内容,针对链篦机篦床温度场变化的特点,本文设计了链篦机温度场的间接测量和带死区的前馈-反馈PID控制算法的间接监控系统,试验运行表明链篦机篦床温度场监控效果良好。
参考词条
补充资料:酶联免疫吸附测定法


酶联免疫吸附测定法


诊法。免疫标记技术之 一。用于测定抗精子抗体。原理为将欲测血清与相应的固相抗原形成免疫复合物,由标记上 酶(过氧化氢酶、碱性磷酸酶、尿素酶等)的二抗检测。利用这结合的酶使基质(邻苯二胺、 对硝基苯酚、尿素等)转化为色素原,出现的颜色反应以目测或分光光度计测出。方法:用 PBS洗涤精子,将其密度调至25×106/50μl。取50μl加到微量滴定盘的每一凹孔中, 以025%戍二醛固定。用含005%Tween20(表面活性剂)的PBS(PBS-Tween)洗3次,加入50 μl稀释的血清或精浆(1∶4稀释),在37℃下孵育60分钟后,用PBS-Tween液将凹孔洗3次, 加入50μl,1∶100稀释的联结有磷性磷酸酶的羊抗人免疫球蛋白,经37℃60分钟孵育,再 用PBS-Tween液洗3次,加入250μl含1mg/ml磷酸对硝基苯酚的005M碳酸钠缓冲液,3 7℃60分钟孵育后,每孔加入50μl3NNaOH以中止反应。用分光光度计在400nm测定吸收光 谱。含磷酸对硝基苯酚和3NNaOH作为空白。本法优点在于不需新鲜精子,适宜大量标本测定 ,但与无关抗原的非特异性反应是主要问题。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。